Restriction map for
pF1KE4294
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# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI AviII BamHI BfrI BsiWI
BsmI BspDI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FseI FspI HpaI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SgrAI SnaBI SpeI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 4192 GACGTC GACGT^C
Acc65I 3782 GGTACC G^GTACC
AccI 802 GTAGAC GT^[AC][GT]AC
AccIII 911 TCCGGA T^CCGGA
AflIII 5354 ACATGT A^C[GA][CT]GT
Alw44I 5913 GTGCAC G^TGCAC
ApaLI 5913 GTGCAC G^TGCAC
Asp718I 3782 GGTACC G^GTACC
AvrII 4263 CCTAGG C^CTAGG
BbrPI 2466 CACGTG CAC^GTG
BbvCI 3864 GCTGAGG CCTCAGC(-5/-2)
BciVI 4863 GGATAC GTATCC(6/5)
BlnI 4263 CCTAGG C^CTAGG
BsaWI 911 TCCGGA [AT]^CCGG[AT]
BseAI 911 TCCGGA T^CCGGA
BsiEI 4528 CGACCG CG[GA][CT]^CG
BsmBI 85 GAGACG CGTCTC(1/5)
Bsp1407I 5456 TGTACA T^GTACA
BspEI 911 TCCGGA T^CCGGA
BspLU11I 5354 ACATGT A^CATGT
BsrGI 5456 TGTACA T^GTACA
BstBI 1102 TTCGAA TT^CGAA
BstEII 136 GGTGACC G^GT[ACGT]ACC
BstPI 136 GGTGACC G^GT[ACGT]ACC
EcoO65I 136 GGTGACC G^GT[ACGT]ACC
EcoRI 4914 GAATTC G^AATTC
HindIII 1292 AAGCTT A^AGCTT
KpnI 3782 GGTACC GGTAC^C
KspI 198 CCGCGG CCGC^GG
MroI 911 TCCGGA T^CCGGA
NspV 1102 TTCGAA TT^CGAA
PmaCI 2466 CACGTG CAC^GTG
PmlI 2466 CACGTG CAC^GTG
SacII 198 CCGCGG CCGC^GG
SfiI 2109 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1102 TTCGAA TT^CGAA
SmaI 209 CCCGGG CCC^GGG
SphI 3926 GCATGC GCATG^C
SspBI 5456 TGTACA T^GTACA
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AhdI 2674 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4156 GACCTGAAGTC
Aor51HI 1589 AGCGCT AGC^GCT
3809 AGCGCT
Asp700I 561 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3894 GAAGAACTTC
AspEI 2674 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4156 GACCTGAAGTC
BcgI 849 CGACCTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2489 GCAGCACCATCG
BfaI 808 CTAG C^TAG
4264 CTAG
BglII 1967 AGATCT A^GATCT
5165 AGATCT
BlpI 1882 GCTCAGC GC^T[ACGT]AGC
4014 GCTCAGC
Bpu1102I 1882 GCTCAGC GC^T[ACGT]AGC
4014 GCTCAGC
BspHI 3668 TCATGA T^CATGA
4493 TCATGA
CelII 1882 GCTCAGC GC^T[ACGT]AGC
4014 GCTCAGC
DraIII 2025 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5934 CACAGAGTG
Eam1105I 2674 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4156 GACCTGAAGTC
Eco47III 1589 AGCGCT AGC^GCT
3809 AGCGCT
HincII 2125 GTCAAC GT[CT]^[GA]AC
5095 GTCAAC
HindII 2125 GTCAAC GT[CT]^[GA]AC
5095 GTCAAC
MaeI 808 CTAG C^TAG
4264 CTAG
NaeI 2046 GCCGGC GCC^GGC
6014 GCCGGC
NgoMIV 2046 GCCGGC G^CCGGC
6014 GCCGGC
RcaI 3668 TCATGA T^CATGA
4493 TCATGA
SexAI 429 ACCAGGT A^CC[AT]GGT
4922 ACCTGGT
SspI 630 AATATT AAT^ATT
2806 AATATT
XmnI 561 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3894 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BclI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScaI ScrFI SfaNI SfcI
SfoI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII