##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwNI         282  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         666  GGTCC                G^G[AT]CC
BbvCI         303  CCTCAGC              CCTCAGC(-5/-2)
BbvI          627  GCAGC                GCAGC(8/12)
BclI          269  TGATCA               T^GATCA
BglII         119  AGATCT               A^GATCT
BpmI          159  CTCCAG               CTGGAG(16/14)
BsaI          156  GGTCTC               GGTCTC(1/5)
BseRI         366  GAGGAG               GAGGAG(10/8)
BsiYI         834  CCTGATTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          834  CCTGATTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI         511  GCAATG               GCAATG(2/0)
BstF5I        831  CATCC                GGATG(2/0)
Cac8I         291  GCTTGC               GC[ACGT]^[ACGT]GC
DraII         402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EarI          504  GAAGAG               CTCTTC(1/4)
EcoO109I      402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         738  GATATC               GAT^ATC
EcoT14I       539  CCATGG               C^C[AT][AT]GG
FbaI          269  TGATCA               T^GATCA
Fnu4HI        627  GCAGC                GC^[ACGT]GC
FokI          831  CATCC                GGATG(9/13)
HaeIII        404  GGCC                 GG^CC
HindIII       677  AAGCTT               A^AGCTT
ItaI          627  GCAGC                GC^[ACGT]GC
Ksp632I       504  GAAGAG               CTCTTC(1/4)
MslI          384  CATTCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          539  CCATGG               C^CATGG
NlaIV         402  GGGGCC               GG[ACGT]^[ACGT]CC
PleI          100  GAGTC                GAGTC(4/5)
SfaNI         624  GATGC                GCATC(5/9)
StyI          539  CCATGG               C^C[AT][AT]GG
TseI          627  GCAGC                G^C[AT]GC
XcmI           29  CCAGTCTGGTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI          154  GCGG                 CCGC(-3/-1)
              459  GCGG                
AlwI          652  GATCC                GGATC(4/5)
              643  GGATC               
BbsI          912  GAAGAC               GAAGAC(2/6)
              778  GTCTTC              
BpuAI         912  GAAGAC               GAAGAC(2/6)
              778  GTCTTC              
BsaJI         539  CCATGG               C^C[ACGT][ACGT]GG
              633  CCGTGG              
BsmAI         111  GAGAC                GTCTC(1/5)
              157  GTCTC               
Cfr13I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
DdeI          304  CTCAG                C^T[ACGT]AG
              552  CTTAG               
DsaI          539  CCATGG               C^C[GA][CT]GG
              633  CCGTGG              
HphI          390  GGTGA                GGTGA(8/7)
              900  GGTGA               
MaeIII         43  GTTAC                ^GT[ACGT]AC
              535  GTAAC               
Sau96I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
TfiI          213  GAATC                G^A[AT]TC
              803  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsrI        
BstYI       DpnII       HinfI       MboI        MboII       
MflI        MnlI        MseI        NdeII       NlaIII      
RsaI        Sau3AI      SmlI        Tru9I       Tsp509I     
TspRI       XhoII