Restriction map for
pF1KE2387
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI AspI AvrII BciVI BclI
BfrI BlnI BsaBI BsiWI Bsp1407I
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I CpoI DraI EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 4600 GACGTC GACGT^C
Acc65I 1061 GGTACC G^GTACC
AflIII 5048 ACATGT A^C[GA][CT]GT
AgeI 1058 ACCGGT A^CCGGT
AhdI 5134 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5046 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1061 GGTACC G^GTACC
AspEI 5134 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BlpI 3270 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3270 GCTGAGC GC^T[ACGT]AGC
BsmI 108 GAATGC GAATGC(1/-1)
BspDI 4123 ATCGAT AT^CGAT
BspLU11I 5048 ACATGT A^CATGT
BsrDI 270 CATTGC GCAATG(2/0)
BstEII 4320 GGTAACC G^GT[ACGT]ACC
BstPI 4320 GGTAACC G^GT[ACGT]ACC
CelII 3270 GCTGAGC GC^T[ACGT]AGC
ClaI 4123 ATCGAT AT^CGAT
DrdI 5206 GACCGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5134 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4320 GGTAACC G^GT[ACGT]ACC
EcoRI 4642 GAATTC G^AATTC
EcoRV 316 GATATC GAT^ATC
KpnI 1061 GGTACC GGTAC^C
KspI 2208 CCGCGG CCGC^GG
MseI 3707 TTAA T^TAA
NheI 3436 GCTAGC G^CTAGC
PinAI 1058 ACCGGT A^CCGGT
PshAI 4832 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2208 CCGCGG CCGC^GG
SexAI 116 ACCAGGT A^CC[AT]GGT
StuI 4086 AGGCCT AGG^CCT
Tru9I 3707 TTAA T^TAA
XmnI 5046 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 3626 TCCGGA T^CCGGA
4448 TCCGGA
AcsI 768 GAATTT [GA]^AATT[CT]
4642 GAATTC
Alw44I 892 GTGCAC G^TGCAC
2110 GTGCAC
ApaI 535 GGGCCC GGGCC^C
3011 GGGCCC
ApaLI 892 GTGCAC G^TGCAC
2110 GTGCAC
ApoI 768 GAATTT [GA]^AATT[CT]
4642 GAATTC
AviII 1007 TGCGCA TGC^GCA
2993 TGCGCA
BbeI 755 GGCGCC GGCGC^C
3073 GGCGCC
BbrPI 2221 CACGTG CAC^GTG
2461 CACGTG
BcgI 4098 CGAGGCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
177 GCAGGAGGGTCG
BseAI 3626 TCCGGA T^CCGGA
4448 TCCGGA
BsiEI 845 CGGCCG CG[GA][CT]^CG
1337 CGGCCG
BsmBI 3926 CGTCTC CGTCTC(1/5)
2957 GAGACG
BspEI 3626 TCCGGA T^CCGGA
4448 TCCGGA
BssSI 1891 CACGAG CACGAG(-5/-1)
4778 CACGAG
DraIII 487 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1217 CACTCTGTG
EagI 845 CGGCCG C^GGCCG
1337 CGGCCG
EclXI 845 CGGCCG C^GGCCG
1337 CGGCCG
Eco52I 845 CGGCCG C^GGCCG
1337 CGGCCG
FspI 1007 TGCGCA TGC^GCA
2993 TGCGCA
HincII 406 GTCAAC GT[CT]^[GA]AC
5215 GTCAAC
HindII 406 GTCAAC GT[CT]^[GA]AC
5215 GTCAAC
KasI 755 GGCGCC G^GCGCC
3073 GGCGCC
MroI 3626 TCCGGA T^CCGGA
4448 TCCGGA
NarI 755 GGCGCC GG^CGCC
3073 GGCGCC
NcoI 1970 CCATGG C^CATGG
3317 CCATGG
PmaCI 2221 CACGTG CAC^GTG
2461 CACGTG
PmlI 2221 CACGTG CAC^GTG
2461 CACGTG
SfoI 755 GGCGCC GGC^GCC
3073 GGCGCC
SmaI 856 CCCGGG CCC^GGG
3077 CCCGGG
SphI 707 GCATGC GCATG^C
4374 GCATGC
XmaCI 856 CCCGGG C^CCGGG
3077 CCCGGG
XmaI 856 CCCGGG C^CCGGG
3077 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SgrAI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII