Restriction map for
pF1KE9669
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# NO CUT ENZYMES ( 48 enzymes) #
##################################
AccIII AclI AgeI AscI AseI
AsnI AspI AviII AvrII BlnI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI FseI FspI MluI
MroI NruI NspV PacI PflFI
PflMI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I
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# ONE CUT ENZYMES ( 22 enzymes) #
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AccI 373 GTCTAC GT^[AC][GT]AC
AflII 6232 CTTAAG C^TTAAG
Alw44I 4409 GTGCAC G^TGCAC
Aor51HI 2270 AGCGCT AGC^GCT
ApaLI 4409 GTGCAC G^TGCAC
BfrI 6232 CTTAAG C^TTAAG
Bsp1407I 4093 TGTACA T^GTACA
BsrBI 2755 GAGCGG CCGCTC(-3/-3)
BsrGI 4093 TGTACA T^GTACA
Eco47III 2270 AGCGCT AGC^GCT
EcoRV 935 GATATC GAT^ATC
HpaI 967 GTTAAC GTT^AAC
KspI 8186 CCGCGG CCGC^GG
MfeI 2943 CAATTG C^AATTG
MunI 2943 CAATTG C^AATTG
NdeI 3995 CATATG CA^TATG
NotI 8188 GCGGCCGC GC^GGCCGC
PshAI 290 GACTCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 8186 CCGCGG CCGC^GG
SexAI 4649 ACCTGGT A^CC[AT]GGT
SfiI 646 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4093 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 40 enzymes) #
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AatII 3563 GACGTC GACGT^C
7593 GACGTC
Acc65I 453 GGTACC G^GTACC
3989 GGTACC
AflIII 5731 ACACGT A^C[GA][CT]GT
8423 ACGTGT
Asp718I 453 GGTACC G^GTACC
3989 GGTACC
BbeI 7007 GGCGCC GGCGC^C
7986 GGCGCC
BciVI 1256 GGATAC GTATCC(6/5)
5932 GTATCC
BglI 647 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
8312 GCCTCATGGGC
BglII 2532 AGATCT A^GATCT
5276 AGATCT
BsaBI 1055 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4456 GATATTGATC
BsiEI 4011 CGGCCG CG[GA][CT]^CG
8189 CGGCCG
BspHI 6900 TCATGA T^CATGA
7577 TCATGA
BstEII 82 GGTTACC G^GT[ACGT]ACC
7620 GGTGACC
BstPI 82 GGTTACC G^GT[ACGT]ACC
7620 GGTGACC
DraI 3805 TTTAAA TTT^AAA
8839 TTTAAA
DrdI 7880 GACATAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8892 GACTGCAGAGTC
EagI 4011 CGGCCG C^GGCCG
8189 CGGCCG
EclXI 4011 CGGCCG C^GGCCG
8189 CGGCCG
Eco52I 4011 CGGCCG C^GGCCG
8189 CGGCCG
EcoO65I 82 GGTTACC G^GT[ACGT]ACC
7620 GGTGACC
EcoT22I 238 ATGCAT ATGCA^T
1141 ATGCAT
KasI 7007 GGCGCC G^GCGCC
7986 GGCGCC
KpnI 453 GGTACC GGTAC^C
3989 GGTACC
MamI 1055 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4456 GATATTGATC
NaeI 513 GCCGGC GCC^GGC
5741 GCCGGC
NarI 7007 GGCGCC GG^CGCC
7986 GGCGCC
NgoMIV 513 GCCGGC G^CCGGC
5741 GCCGGC
NheI 5514 GCTAGC G^CTAGC
8661 GCTAGC
NsiI 238 ATGCAT ATGCA^T
1141 ATGCAT
PaeR7I 5027 CTCGAG C^TCGAG
5831 CTCGAG
RcaI 6900 TCATGA T^CATGA
7577 TCATGA
SacI 5846 GAGCTC GAGCT^C
7093 GAGCTC
SapI 3514 GAAGAGC GCTCTTC(1/4)
8935 GAAGAGC
SfoI 7007 GGCGCC GGC^GCC
7986 GGCGCC
SmaI 1560 CCCGGG CCC^GGG
8697 CCCGGG
SphI 383 GCATGC GCATG^C
5228 GCATGC
XbaI 1686 TCTAGA T^CTAGA
6787 TCTAGA
XcmI 342 CCATGGTGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1494 CCAACAACATCCTGG
XhoI 5027 CTCGAG C^TCGAG
5831 CTCGAG
XmaCI 1560 CCCGGG C^CCGGG
8697 CCCGGG
XmaI 1560 CCCGGG C^CCGGG
8697 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI