Restriction map for
pF1KE2373
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BcgI
BclI BfrI BglII BlnI BsiWI
BsmI BspDI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoRI EcoRV FbaI FseI
FspI HincII HindII HpaI KpnI
MfeI MseI MunI NaeI NcoI
NdeI NgoMIV NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 1556 AAATTC [GA]^AATT[CT]
Alw44I 624 GTGCAC G^TGCAC
ApaLI 624 GTGCAC G^TGCAC
ApoI 1556 AAATTC [GA]^AATT[CT]
Asp700I 3375 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1047 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2545 CCCGAG C^[CT]CG[GA]G
BamHI 1352 GGATCC G^GATCC
BbrPI 3424 CACGTG CAC^GTG
BciVI 2913 GGATAC GTATCC(6/5)
BlpI 2360 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2360 GCTCAGC GC^T[ACGT]AGC
BsaAI 3424 CACGTG [CT]AC^GT[GA]
BsaBI 1773 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1897 CGGCCG CG[GA][CT]^CG
BsoBI 2545 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3173 TGTACA T^GTACA
BspLU11I 3026 ACATGT A^CATGT
BsrGI 3173 TGTACA T^GTACA
BssSI 2182 CACGAG CACGAG(-5/-1)
CelII 2360 GCTCAGC GC^T[ACGT]AGC
DraIII 532 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1897 CGGCCG C^GGCCG
EclXI 1897 CGGCCG C^GGCCG
Eco52I 1897 CGGCCG C^GGCCG
EcoNI 2946 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1305 ATGCAT ATGCA^T
HindIII 2320 AAGCTT A^AGCTT
MamI 1773 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 103 ACGCGT A^CGCGT
NotI 1896 GCGGCCGC GC^GGCCGC
NsiI 1305 ATGCAT ATGCA^T
PflFI 1047 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3424 CACGTG CAC^GTG
PmlI 3424 CACGTG CAC^GTG
SphI 3222 GCATGC GCATG^C
SspBI 3173 TGTACA T^GTACA
Tth111I 1047 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3375 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1298 TCCGGA T^CCGGA
1372 TCCGGA
AocI 1086 CCTGAGG CC^T[ACGT]AGG
1123 CCTGAGG
Aor51HI 273 AGCGCT AGC^GCT
1217 AGCGCT
BbeI 2051 GGCGCC GGCGC^C
2506 GGCGCC
BseAI 1298 TCCGGA T^CCGGA
1372 TCCGGA
BsmBI 100 GAGACG CGTCTC(1/5)
2597 GAGACG
BspEI 1298 TCCGGA T^CCGGA
1372 TCCGGA
BsrDI 1323 CATTGC GCAATG(2/0)
971 GCAATG
BstAPI 1416 GCAACGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1991 GCAAGTGGTGC
BstEII 1135 GGTGACC G^GT[ACGT]ACC
1918 GGTGACC
BstPI 1135 GGTGACC G^GT[ACGT]ACC
1918 GGTGACC
BstYI 716 AGATCC [GA]^GATC[CT]
1352 GGATCC
Bsu36I 1086 CCTGAGG CC^T[ACGT]AGG
1123 CCTGAGG
DrdI 910 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1138 GACCTCCTCGTC
Eco47III 273 AGCGCT AGC^GCT
1217 AGCGCT
Eco81I 1086 CCTGAGG CC^T[ACGT]AGG
1123 CCTGAGG
EcoO65I 1135 GGTGACC G^GT[ACGT]ACC
1918 GGTGACC
EcoT14I 71 CCTTGG C^C[AT][AT]GG
2930 CCAAGG
KasI 2051 GGCGCC G^GCGCC
2506 GGCGCC
KspI 377 CCGCGG CCGC^GG
1075 CCGCGG
MflI 716 AGATCC [GA]^GATC[CT]
1352 GGATCC
MroI 1298 TCCGGA T^CCGGA
1372 TCCGGA
NarI 2051 GGCGCC GG^CGCC
2506 GGCGCC
NheI 1737 GCTAGC G^CTAGC
2787 GCTAGC
NspI 3026 ACATGT [GA]CATG^[CT]
3222 GCATGC
PflMI 534 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3280 CCACAGGATGG
PshAI 1332 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3333 GACATCTGTC
SacII 377 CCGCGG CCGC^GG
1075 CCGCGG
SexAI 533 ACCAGGT A^CC[AT]GGT
2672 ACCTGGT
SfiI 1468 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1477 GGCCGCACGGGCC
SfoI 2051 GGCGCC GGC^GCC
2506 GGCGCC
SmlI 217 CTCAAG C^T[CT][GA]AG
913 CTCAAG
StuI 68 AGGCCT AGG^CCT
3365 AGGCCT
StyI 71 CCTTGG C^C[AT][AT]GG
2930 CCAAGG
Van91I 534 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3280 CCACAGGATGG
XhoII 716 AGATCC [GA]^GATC[CT]
1352 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI