Restriction map for
pF1KB6207
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AlwNI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmBI BspDI BspHI BspMI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1608 CGCG CG^CG
AccIII 1544 TCCGGA T^CCGGA
AcyI 799 GGCGTC G[GA]^CG[CT]C
AflIII 1821 ACATGT A^C[GA][CT]GT
AhdI 1611 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1567 GTGCAC G^TGCAC
ApaI 1981 GGGCCC GGGCC^C
ApaLI 1567 GTGCAC G^TGCAC
AspEI 1611 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 497 TGGCCA TGG^CCA
BanI 336 GGTGCC G^G[CT][GA]CC
BbvCI 907 CCTCAGC CCTCAGC(-5/-2)
BciVI 1577 GGATAC GTATCC(6/5)
BclI 729 TGATCA T^GATCA
BfaI 854 CTAG C^TAG
BglI 718 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 799 GGCGTC G[GA]^CG[CT]C
BseAI 1544 TCCGGA T^CCGGA
BspEI 1544 TCCGGA T^CCGGA
BspLU11I 1821 ACATGT A^CATGT
BsrDI 53 CATTGC GCAATG(2/0)
BsrFI 380 ACCGGC [GA]^CCGG[CT]
BssSI 1293 CACGAG CACGAG(-5/-1)
BstAPI 330 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1608 CGCG CG^CG
Cfr10I 380 ACCGGC [GA]^CCGG[CT]
DraIII 1832 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 793 GACAGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1611 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1289 CCTTCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 989 GAATTC G^AATTC
FauI 1784 GCGGG CCCGC(4/6)
FbaI 729 TGATCA T^GATCA
HgaI 800 GCGTC GACGC(5/10)
Hin1I 799 GGCGTC G[GA]^CG[CT]C
HincII 833 GTCAAC GT[CT]^[GA]AC
HindII 833 GTCAAC GT[CT]^[GA]AC
MaeI 854 CTAG C^TAG
MluNI 497 TGGCCA TGG^CCA
MroI 1544 TCCGGA T^CCGGA
MscI 497 TGGCCA TGG^CCA
MvnI 1608 CGCG CG^CG
NcoI 490 CCATGG C^CATGG
NdeI 1514 CATATG CA^TATG
PaeR7I 371 CTCGAG C^TCGAG
PflMI 1972 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 109 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1166 CTGCAG CTGCA^G
SphI 1771 GCATGC GCATG^C
SspI 1211 AATATT AAT^ATT
Van91I 1972 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 371 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 989 GAATTC [GA]^AATT[CT]
1032 AAATTC
AlwI 876 GATCC GGATC(4/5)
267 GGATC
ApoI 989 GAATTC [GA]^AATT[CT]
1032 AAATTC
AspHI 1551 GAGCAC G[AT]GC[AT]^C
1567 GTGCAC
AvaI 371 CTCGAG C^[CT]CG[GA]G
1066 CCCGAG
BanII 1717 GAGCCC G[GA]GC[CT]^C
1981 GGGCCC
BbrPI 521 CACGTG CAC^GTG
1402 CACGTG
BcnI 310 CCGGG CC^[GC]GG
1841 CCGGG
BsaAI 521 CACGTG [CT]AC^GT[GA]
1402 CACGTG
BsaWI 1249 ACCGGA [AT]^CCGG[AT]
1544 TCCGGA
BsiHKAI 1551 GAGCAC G[AT]GC[AT]^C
1567 GTGCAC
BsmFI 293 GGGAC GGGAC(10/14)
423 GGGAC
BsoBI 371 CTCGAG C^[CT]CG[GA]G
1066 CCCGAG
Bsp1407I 1818 TGTACA T^GTACA
1902 TGTACA
BsrGI 1818 TGTACA T^GTACA
1902 TGTACA
BstXI 640 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1692 CCAGGAATGTGG
BstYI 267 GGATCT [GA]^GATC[CT]
875 AGATCC
CfoI 65 GCGC GCG^C
1607 GCGC
DraII 109 AGGACCC [GA]G^G[ACGT]CC[CT]
1981 GGGCCCC
DsaI 29 CCGTGG C^C[GA][CT]GG
490 CCATGG
EcoO109I 109 AGGACCC [GA]G^G[ACGT]CC[CT]
1981 GGGCCCC
EcoT14I 403 CCTTGG C^C[AT][AT]GG
490 CCATGG
EcoT22I 1097 ATGCAT ATGCA^T
1769 ATGCAT
HhaI 65 GCGC GCG^C
1607 GCGC
HinP1I 65 GCGC G^CGC
1607 GCGC
MflI 267 GGATCT [GA]^GATC[CT]
875 AGATCC
MspA1I 160 CAGCTG C[AC]G^C[GT]G
331 CAGCTG
NciI 310 CCGGG CC^[GC]GG
1841 CCGGG
NsiI 1097 ATGCAT ATGCA^T
1769 ATGCAT
PmaCI 521 CACGTG CAC^GTG
1402 CACGTG
PmlI 521 CACGTG CAC^GTG
1402 CACGTG
PvuII 160 CAGCTG CAG^CTG
331 CAGCTG
SfcI 705 CTACAG C^T[GA][CT]AG
1166 CTGCAG
SspBI 1818 TGTACA T^GTACA
1902 TGTACA
StyI 403 CCTTGG C^C[AT][AT]GG
490 CCATGG
TaqI 372 TCGA T^CGA
1219 TCGA
TthHB8I 372 TCGA T^CGA
1219 TCGA
XhoII 267 GGATCT [GA]^GATC[CT]
875 AGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AocI Asp700I
AvaII BbsI BbvI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII EaeI
EarI Eco81I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI