Restriction map for
pF1KE0764
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 714 AACGTT AA^CGTT
AcsI 1302 AAATTC [GA]^AATT[CT]
AflIII 386 ACGTGT A^C[GA][CT]GT
AlwNI 1132 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1302 AAATTC [GA]^AATT[CT]
Asp700I 186 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 29 GTGCTC G[AT]GC[AT]^C
AspI 522 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 987 GGACC G^G[AT]CC
BalI 1118 TGGCCA TGG^CCA
BbsI 612 GAAGAC GAAGAC(2/6)
BglII 111 AGATCT A^GATCT
BmyI 29 GTGCTC G[AGT]GC[ACT]^C
BpuAI 612 GAAGAC GAAGAC(2/6)
BsiHKAI 29 GTGCTC G[AT]GC[AT]^C
BsiYI 1131 CCAGTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1131 CCAGTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 301 GAATGC GAATGC(1/-1)
Bsp1286I 29 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 947 TGTACA T^GTACA
BspMI 269 ACCTGC ACCTGC(4/8)
BsrGI 947 TGTACA T^GTACA
DraII 767 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 912 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1118 TGGCCA [CT]^GGCC[GA]
EcoO109I 767 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 99 GATATC GAT^ATC
EcoT14I 132 CCAAGG C^C[AT][AT]GG
FauI 1224 GCGGG CCCGC(4/6)
HgaI 45 GCGTC GACGC(5/10)
HpaI 673 GTTAAC GTT^AAC
MluNI 1118 TGGCCA TGG^CCA
MscI 1118 TGGCCA TGG^CCA
NlaIV 986 GGGACC GG[ACGT]^[ACGT]CC
NspI 315 GCATGC [GA]CATG^[CT]
PflFI 522 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1131 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 714 AACGTT AA^CGTT
PvuII 362 CAGCTG CAG^CTG
SphI 315 GCATGC GCATG^C
SspBI 947 TGTACA T^GTACA
StyI 132 CCAAGG C^C[AT][AT]GG
TaqI 1000 TCGA T^CGA
TfiI 427 GAATC G^A[AT]TC
Tsp509I 1303 AATT ^AATT
Tth111I 522 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1000 TCGA T^CGA
Van91I 1131 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 186 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BfaI 821 CTAG C^TAG
968 CTAG
BsgI 536 CTGCAC GTGCAG(16/14)
373 GTGCAG
BspHI 852 TCATGA T^CATGA
1184 TCATGA
BsrI 224 ACTGG ACTGG(1/-1)
1131 CCAGT
BstYI 111 AGATCT [GA]^GATC[CT]
474 AGATCC
EarI 591 CTCTTC CTCTTC(1/4)
907 GAAGAG
HincII 265 GTCAAC GT[CT]^[GA]AC
673 GTTAAC
HindII 265 GTCAAC GT[CT]^[GA]AC
673 GTTAAC
Ksp632I 591 CTCTTC CTCTTC(1/4)
907 GAAGAG
MaeI 821 CTAG C^TAG
968 CTAG
MflI 111 AGATCT [GA]^GATC[CT]
474 AGATCC
MslI 296 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1094 CATTAAAGTG
MspA1I 362 CAGCTG C[AC]G^C[GT]G
1222 CAGCGG
PleI 522 GACTC GAGTC(4/5)
87 GAGTC
RcaI 852 TCATGA T^CATGA
1184 TCATGA
SmlI 68 CTCAAG C^T[CT][GA]AG
1270 CTCAAG
Tsp45I 216 GTCAC ^GT[GC]AC
787 GTCAC
XhoII 111 AGATCT [GA]^GATC[CT]
474 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BseRI BsmAI BsmFI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
TspRI