Restriction map for
pF1KB5949
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 1384 GTAC GT^AC
AflIII 1464 ACATGT A^C[GA][CT]GT
Alw44I 683 GTGCAC G^TGCAC
ApaLI 683 GTGCAC G^TGCAC
AseI 504 ATTAAT AT^TAAT
AsnI 504 ATTAAT AT^TAAT
Asp700I 173 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 683 GTGCAC G[AT]GC[AT]^C
BanII 396 GGGCTC G[GA]GC[CT]^C
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
BcgI 513 CGAACCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 63 CCGGG CC^[GC]GG
BglI 37 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1047 AGATCT A^GATCT
BsaAI 1418 TACGTG [CT]AC^GT[GA]
BsiHKAI 683 GTGCAC G[AT]GC[AT]^C
BsiYI 1149 CCTGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1149 CCTGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 1464 ACATGT A^CATGT
BsrFI 88 GCCGGT [GA]^CCGG[CT]
BstAPI 79 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 91 GGTGACC G^GT[ACGT]ACC
BstPI 91 GGTGACC G^GT[ACGT]ACC
CfoI 763 GCGC GCG^C
Cfr10I 88 GCCGGT [GA]^CCGG[CT]
EcoO65I 91 GGTGACC G^GT[ACGT]ACC
EcoRI 1127 GAATTC G^AATTC
EcoRV 982 GATATC GAT^ATC
EcoT14I 821 CCTTGG C^C[AT][AT]GG
HgaI 617 GCGTC GACGC(5/10)
HhaI 763 GCGC GCG^C
HinP1I 763 GCGC G^CGC
HindIII 589 AAGCTT A^AGCTT
MaeII 1419 ACGT A^CGT
NciI 63 CCGGG CC^[GC]GG
NlaIV 1222 GGAGCC GG[ACGT]^[ACGT]CC
PshBI 504 ATTAAT AT^TAAT
PstI 521 CTGCAG CTGCA^G
RsaI 1384 GTAC GT^AC
SapI 526 GCTCTTC GCTCTTC(1/4)
StyI 821 CCTTGG C^C[AT][AT]GG
XmnI 173 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 252 CGCG CG^CG
764 CGCG
AciI 4 GCGG CCGC(-3/-1)
253 GCGG
AcsI 325 AAATTT [GA]^AATT[CT]
1127 GAATTC
AlwNI 205 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1033 CAGCAACTG
ApoI 325 AAATTT [GA]^AATT[CT]
1127 GAATTC
BfaI 476 CTAG C^TAG
715 CTAG
BmyI 396 GGGCTC G[AGT]GC[ACT]^C
683 GTGCAC
BsgI 27 CTGCAC GTGCAG(16/14)
1109 GTGCAG
BsmFI 1255 GGGAC GGGAC(10/14)
427 GTCCC
Bsp1286I 396 GGGCTC G[AGT]GC[ACT]^C
683 GTGCAC
BsrDI 1286 CATTGC GCAATG(2/0)
878 GCAATG
BstUI 252 CGCG CG^CG
764 CGCG
EarI 527 CTCTTC CTCTTC(1/4)
133 GAAGAG
HapII 63 CCGG C^CGG
89 CCGG
HpaI 331 GTTAAC GTT^AAC
628 GTTAAC
HpaII 63 CCGG C^CGG
89 CCGG
HphI 91 GGTGA GGTGA(8/7)
18 TCACC
Ksp632I 527 CTCTTC CTCTTC(1/4)
133 GAAGAG
MaeI 476 CTAG C^TAG
715 CTAG
MspA1I 107 CAGCTG C[AC]G^C[GT]G
1058 CAGCTG
MspI 63 CCGG C^CGG
89 CCGG
MvnI 252 CGCG CG^CG
764 CGCG
NspI 1464 ACATGT [GA]CATG^[CT]
1471 ACATGC
PvuII 107 CAGCTG CAG^CTG
1058 CAGCTG
SspI 175 AATATT AAT^ATT
574 AATATT
TaqI 1017 TCGA T^CGA
1272 TCGA
Tsp45I 92 GTGAC ^GT[GC]AC
1468 GTGAC
TthHB8I 1017 TCGA T^CGA
1272 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AlwI BbsI BbvI BpmI
BpuAI BsaJI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI ItaI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII PleI
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XhoII