Restriction map for
pF1KB9185
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 99 GTGCAC G^TGCAC
ApaLI 99 GTGCAC G^TGCAC
AvaI 496 CCCGAG C^[CT]CG[GA]G
BamHI 725 GGATCC G^GATCC
BanI 479 GGCGCC G^G[CT][GA]CC
BanII 348 GAGCTC G[GA]GC[CT]^C
BbeI 479 GGCGCC GGCGC^C
BbvCI 474 GCTGAGG CCTCAGC(-5/-2)
BsaAI 382 TACGTG [CT]AC^GT[GA]
BsaI 544 GAGACC GGTCTC(1/5)
BsoBI 496 CCCGAG C^[CT]CG[GA]G
Bsp1407I 217 TGTACA T^GTACA
BsrFI 117 GCCGGT [GA]^CCGG[CT]
BsrGI 217 TGTACA T^GTACA
BstYI 725 GGATCC [GA]^GATC[CT]
Cfr10I 117 GCCGGT [GA]^CCGG[CT]
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 210 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 457 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 115 TGGCCG [CT]^GGCC[GA]
EarI 964 CTCTTC CTCTTC(1/4)
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 789 CCCGC CCCGC(4/6)
KasI 479 GGCGCC G^GCGCC
Ksp632I 964 CTCTTC CTCTTC(1/4)
KspI 616 CCGCGG CCGC^GG
MflI 725 GGATCC [GA]^GATC[CT]
NarI 479 GGCGCC GG^CGCC
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 348 GAGCTC GAGCT^C
SacII 616 CCGCGG CCGC^GG
SfoI 479 GGCGCC GGC^GCC
SmlI 598 CTCAAG C^T[CT][GA]AG
SspBI 217 TGTACA T^GTACA
XhoII 725 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 479 GGCGCC G[GA]^CG[CT]C
982 GGCGTC
AflIII 503 ACATGT A^C[GA][CT]GT
755 ACATGT
Aor51HI 248 AGCGCT AGC^GCT
329 AGCGCT
BsaHI 479 GGCGCC G[GA]^CG[CT]C
982 GGCGTC
BseRI 445 GAGGAG GAGGAG(10/8)
765 GAGGAG
BsgI 37 CTGCAC GTGCAG(16/14)
207 CTGCAC
BsmAI 544 GAGAC GTCTC(1/5)
957 GTCTC
BspLU11I 503 ACATGT A^CATGT
755 ACATGT
BspMI 41 ACCTGC ACCTGC(4/8)
608 ACCTGC
BsrI 180 ACTGG ACTGG(1/-1)
27 CCAGT
Eco47III 248 AGCGCT AGC^GCT
329 AGCGCT
EcoT14I 353 CCAAGG C^C[AT][AT]GG
623 CCAAGG
Hin1I 479 GGCGCC G[GA]^CG[CT]C
982 GGCGTC
MaeII 383 ACGT A^CGT
403 ACGT
MaeIII 682 GTAAC ^GT[ACGT]AC
1072 GTAAC
MseI 896 TTAA T^TAA
1115 TTAA
MslI 564 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATGTGCGTG
PvuII 94 CAGCTG CAG^CTG
668 CAGCTG
StyI 353 CCAAGG C^C[AT][AT]GG
623 CCAAGG
Tru9I 896 TTAA T^TAA
1115 TTAA
TspRI 221 CACTG CA[GC]TG[ACGT][ACGT]^
814 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI TaqI TseI
TthHB8I