Restriction map for
pF1KE1072
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eco81I
EcoNI EcoRV EcoT22I FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 585 CGCG CG^CG
AcyI 304 GGCGTC G[GA]^CG[CT]C
ApaI 2164 GGGCCC GGGCC^C
AvaI 2048 CTCGAG C^[CT]CG[GA]G
BbsI 2173 GAAGAC GAAGAC(2/6)
BclI 1820 TGATCA T^GATCA
BcnI 29 CCCGG CC^[GC]GG
BglI 1646 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2173 GAAGAC GAAGAC(2/6)
BsaAI 2073 CACGTA [CT]AC^GT[GA]
BsaBI 1230 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 304 GGCGTC G[GA]^CG[CT]C
BsaWI 1967 TCCGGT [AT]^CCGG[AT]
BsmI 1361 GCATTC GAATGC(1/-1)
BsoBI 2048 CTCGAG C^[CT]CG[GA]G
BspHI 683 TCATGA T^CATGA
BspLU11I 1438 ACATGT A^CATGT
BsrFI 1664 ACCGGC [GA]^CCGG[CT]
BstEII 1692 GGTGACC G^GT[ACGT]ACC
BstPI 1692 GGTGACC G^GT[ACGT]ACC
BstUI 585 CGCG CG^CG
Cfr10I 1664 ACCGGC [GA]^CCGG[CT]
DraIII 1687 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1692 GGTGACC G^GT[ACGT]ACC
EcoRI 624 GAATTC G^AATTC
FbaI 1820 TGATCA T^GATCA
HgaI 305 GCGTC GACGC(5/10)
Hin1I 304 GGCGTC G[GA]^CG[CT]C
HindIII 1385 AAGCTT A^AGCTT
MamI 1230 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 585 CGCG CG^CG
NciI 29 CCCGG CC^[GC]GG
NcoI 1088 CCATGG C^CATGG
NdeI 896 CATATG CA^TATG
NheI 144 GCTAGC G^CTAGC
PaeR7I 2048 CTCGAG C^TCGAG
PflMI 1955 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1846 CTGCAG CTGCA^G
PvuII 192 CAGCTG CAG^CTG
RcaI 683 TCATGA T^CATGA
SexAI 252 ACCTGGT A^CC[AT]GGT
SspI 1579 AATATT AAT^ATT
StuI 693 AGGCCT AGG^CCT
Van91I 1955 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1001 CCAAATTCGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2048 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 845 ACACGT A^C[GA][CT]GT
1438 ACATGT
AhdI 251 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1034 GACTGACAGTC
Aor51HI 1185 AGCGCT AGC^GCT
2044 AGCGCT
AspEI 251 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1034 GACTGACAGTC
BalI 233 TGGCCA TGG^CCA
1013 TGGCCA
BamHI 74 GGATCC G^GATCC
1964 GGATCC
BanI 930 GGTGCC G^G[CT][GA]CC
1534 GGCACC
BanII 1102 GGGCTC G[GA]GC[CT]^C
2164 GGGCCC
BsgI 2062 CTGCAC GTGCAG(16/14)
37 GTGCAG
BsiEI 443 CGGCCG CG[GA][CT]^CG
2220 CGGCCG
BsrDI 276 CATTGC GCAATG(2/0)
61 GCAATG
CfoI 1186 GCGC GCG^C
2045 GCGC
EagI 443 CGGCCG C^GGCCG
2220 CGGCCG
Eam1105I 251 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1034 GACTGACAGTC
EclXI 443 CGGCCG C^GGCCG
2220 CGGCCG
Eco47III 1185 AGCGCT AGC^GCT
2044 AGCGCT
Eco52I 443 CGGCCG C^GGCCG
2220 CGGCCG
FauI 33 GCGGG CCCGC(4/6)
2162 GCGGG
HaeII 1185 AGCGCT [GA]GCGC^[CT]
2044 AGCGCT
HhaI 1186 GCGC GCG^C
2045 GCGC
HinP1I 1186 GCGC G^CGC
2045 GCGC
HincII 1503 GTCAAC GT[CT]^[GA]AC
1516 GTCAAC
HindII 1503 GTCAAC GT[CT]^[GA]AC
1516 GTCAAC
MluNI 233 TGGCCA TGG^CCA
1013 TGGCCA
MscI 233 TGGCCA TGG^CCA
1013 TGGCCA
MseI 919 TTAA T^TAA
1597 TTAA
MspA1I 192 CAGCTG C[AC]G^C[GT]G
501 CCGCTG
PpuMI 249 AGGACCT [GA]G^G[AT]CC[CT]
1054 GGGTCCC
SapI 2241 GAAGAGC GCTCTTC(1/4)
1713 GCTCTTC
SfcI 363 CTACAG C^T[GA][CT]AG
1846 CTGCAG
SmlI 1209 CTTGAG C^T[CT][GA]AG
2048 CTCGAG
Tru9I 919 TTAA T^TAA
1597 TTAA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII