Restriction map for
pF1KE1071
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 153 GGTACC G^GTACC
Alw44I 883 GTGCAC G^TGCAC
ApaI 245 GGGCCC GGGCC^C
ApaLI 883 GTGCAC G^TGCAC
Asp700I 2137 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 153 GGTACC G^GTACC
AspHI 883 GTGCAC G[AT]GC[AT]^C
AspI 1099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1618 TGGCCA TGG^CCA
BanI 153 GGTACC G^G[CT][GA]CC
BanII 245 GGGCCC G[GA]GC[CT]^C
BbvCI 217 CCTCAGC CCTCAGC(-5/-2)
BciVI 574 GTATCC GTATCC(6/5)
BglII 348 AGATCT A^GATCT
BsaI 734 GAGACC GGTCTC(1/5)
BsgI 2009 CTGCAC GTGCAG(16/14)
BsiHKAI 883 GTGCAC G[AT]GC[AT]^C
BsmI 971 GAATGC GAATGC(1/-1)
BsrBI 1857 CCGCTC CCGCTC(-3/-3)
BstXI 1869 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2248 GCGC GCG^C
DraI 870 TTTAAA TTT^AAA
DraIII 223 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1618 TGGCCA [CT]^GGCC[GA]
EcoNI 249 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2270 CCAAGG C^C[AT][AT]GG
HaeII 2247 GGCGCT [GA]GCGC^[CT]
HhaI 2248 GCGC GCG^C
HinP1I 2248 GCGC G^CGC
HpaI 474 GTTAAC GTT^AAC
KpnI 153 GGTACC GGTAC^C
MaeII 2061 ACGT A^CGT
MluNI 1618 TGGCCA TGG^CCA
MscI 1618 TGGCCA TGG^CCA
MslI 860 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 315 CAGCGG C[AC]G^C[GT]G
NspI 913 GCATGT [GA]CATG^[CT]
PflFI 1099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 654 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 130 GGGACCC [GA]G^G[AT]CC[CT]
SapI 1930 GAAGAGC GCTCTTC(1/4)
ScaI 896 AGTACT AGT^ACT
StyI 2270 CCAAGG C^C[AT][AT]GG
TaqI 2124 TCGA T^CGA
Tth111I 1099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2124 TCGA T^CGA
Van91I 654 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2102 CCAAACTGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2137 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 1244 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1369 CAGTTACTG
AvaI 201 CCCGGG C^[CT]CG[GA]G
309 CCCGGG
BfaI 1841 CTAG C^TAG
2072 CTAG
BsaBI 832 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1006 GATTTTTATC
BseRI 17 GAGGAG GAGGAG(10/8)
231 GAGGAG
BsoBI 201 CCCGGG C^[CT]CG[GA]G
309 CCCGGG
Cac8I 209 GCCTGC GC[ACGT]^[ACGT]GC
259 GCGGGC
DraII 130 GGGACCC [GA]G^G[ACGT]CC[CT]
245 GGGCCCT
DsaI 193 CCGTGG C^C[GA][CT]GG
1615 CCGTGG
EcoO109I 130 GGGACCC [GA]G^G[ACGT]CC[CT]
245 GGGCCCT
MaeI 1841 CTAG C^TAG
2072 CTAG
MamI 832 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1006 GATTTTTATC
PstI 211 CTGCAG CTGCA^G
466 CTGCAG
SfcI 211 CTGCAG C^T[GA][CT]AG
466 CTGCAG
SmaI 201 CCCGGG CCC^GGG
309 CCCGGG
SmlI 1118 CTCAAG C^T[CT][GA]AG
1357 CTTGAG
SspI 904 AATATT AAT^ATT
1685 AATATT
XmaCI 201 CCCGGG C^CCGGG
309 CCCGGG
XmaI 201 CCCGGG C^CCGGG
309 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII