Restriction map for
pF1KE1074
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BcnI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI FseI FspI
HaeIII HapII HgaI Hin1I HpaI
HpaII KasI KpnI MamI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1405 GTCTAC GT^[AC][GT]AC
AccII 1697 CGCG CG^CG
AflII 973 CTTAAG C^TTAAG
AflIII 1040 ACATGT A^C[GA][CT]GT
Alw44I 1786 GTGCAC G^TGCAC
AlwNI 1552 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 599 AGCGCT AGC^GCT
ApaLI 1786 GTGCAC G^TGCAC
BamHI 1338 GGATCC G^GATCC
BanII 63 GGGCTC G[GA]GC[CT]^C
BbvCI 762 GCTGAGG CCTCAGC(-5/-2)
BfrI 973 CTTAAG C^TTAAG
BglII 1921 AGATCT A^GATCT
BlpI 1743 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1743 GCTGAGC GC^T[ACGT]AGC
BsgI 751 GTGCAG GTGCAG(16/14)
BspHI 408 TCATGA T^CATGA
BspLU11I 1040 ACATGT A^CATGT
BstEII 768 GGTGACC G^GT[ACGT]ACC
BstPI 768 GGTGACC G^GT[ACGT]ACC
BstUI 1697 CGCG CG^CG
Cac8I 61 GCGGGC GC[ACGT]^[ACGT]GC
CelII 1743 GCTGAGC GC^T[ACGT]AGC
Eco47III 599 AGCGCT AGC^GCT
EcoO65I 768 GGTGACC G^GT[ACGT]ACC
HindIII 721 AAGCTT A^AGCTT
KspI 1696 CCGCGG CCGC^GG
MaeII 780 ACGT A^CGT
MfeI 569 CAATTG C^AATTG
MunI 569 CAATTG C^AATTG
MvnI 1697 CGCG CG^CG
NcoI 1122 CCATGG C^CATGG
NdeI 655 CATATG CA^TATG
NspI 1040 ACATGT [GA]CATG^[CT]
PflMI 1182 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 343 GACTC GAGTC(4/5)
PvuII 1690 CAGCTG CAG^CTG
RcaI 408 TCATGA T^CATGA
SacII 1696 CCGCGG CCGC^GG
SapI 2092 GCTCTTC GCTCTTC(1/4)
ScaI 1050 AGTACT AGT^ACT
SfcI 1883 CTACAG C^T[GA][CT]AG
SpeI 885 ACTAGT A^CTAGT
Van91I 1182 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 1451 GTGCTC G[AT]GC[AT]^C
1786 GTGCAC
AvaII 1107 GGTCC G^G[AT]CC
1311 GGACC
BciVI 1629 GGATAC GTATCC(6/5)
1869 GGATAC
BclI 891 TGATCA T^GATCA
1145 TGATCA
BpmI 1174 CTCCAG CTGGAG(16/14)
985 CTGGAG
BsiHKAI 1451 GTGCTC G[AT]GC[AT]^C
1786 GTGCAC
BsiYI 68 CCTTTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1182 CCATAATTTGG
BslI 68 CCTTTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1182 CCATAATTTGG
BsmAI 354 GTCTC GTCTC(1/5)
415 GTCTC
BstAPI 753 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
961 GCACCGATTGC
CfoI 526 GCGC GCG^C
600 GCGC
Cfr13I 1107 GGTCC G^G[ACGT]CC
1311 GGACC
DraI 180 TTTAAA TTT^AAA
250 TTTAAA
DsaI 1122 CCATGG C^C[GA][CT]GG
1696 CCGCGG
EcoRV 214 GATATC GAT^ATC
224 GATATC
EcoT22I 239 ATGCAT ATGCA^T
512 ATGCAT
FauI 61 GCGGG CCCGC(4/6)
1698 GCGGG
FbaI 891 TGATCA T^GATCA
1145 TGATCA
HaeII 525 AGCGCC [GA]GCGC^[CT]
599 AGCGCT
HhaI 526 GCGC GCG^C
600 GCGC
HinP1I 526 GCGC G^CGC
600 GCGC
HincII 3 GTTGAC GT[CT]^[GA]AC
819 GTCAAC
HindII 3 GTTGAC GT[CT]^[GA]AC
819 GTCAAC
MspA1I 1690 CAGCTG C[AC]G^C[GT]G
1696 CCGCGG
NsiI 239 ATGCAT ATGCA^T
512 ATGCAT
Sau96I 1107 GGTCC G^G[ACGT]CC
1311 GGACC
SmlI 973 CTTAAG C^T[CT][GA]AG
999 CTTGAG
TaqI 1232 TCGA T^CGA
1293 TCGA
TspRI 35 CAGTG CA[GC]TG[ACGT][ACGT]^
1575 CAGTG
TthHB8I 1232 TCGA T^CGA
1293 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I XhoII
XmnI