################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI HapII HgaI HincII HindII HpaI HpaII KasI KpnI KspI MluI MluNI MroI MscI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 1183 GACGTC GACGT^C AccI 1336 GTCTAC GT^[AC][GT]AC AcyI 1183 GACGTC G[GA]^CG[CT]C AflIII 737 ACATGT A^C[GA][CT]GT AocI 615 CCTAAGG CC^T[ACGT]AGG Aor51HI 532 AGCGCT AGC^GCT ApaI 1385 GGGCCC GGGCC^C AspI 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 1385 GGGCCC G[GA]GC[CT]^C BclI 18 TGATCA T^GATCA BglII 1305 AGATCT A^GATCT BmyI 1385 GGGCCC G[AGT]GC[ACT]^C BsaHI 1183 GACGTC G[GA]^CG[CT]C BseRI 276 GAGGAG GAGGAG(10/8) Bsp1286I 1385 GGGCCC G[AGT]GC[ACT]^C BspLU11I 737 ACATGT A^CATGT BsrBI 1381 GAGCGG CCGCTC(-3/-3) BstEII 285 GGTGACC G^GT[ACGT]ACC BstPI 285 GGTGACC G^GT[ACGT]ACC BstYI 1305 AGATCT [GA]^GATC[CT] Bsu36I 615 CCTAAGG CC^T[ACGT]AGG CfoI 533 GCGC GCG^C Eco47III 532 AGCGCT AGC^GCT Eco81I 615 CCTAAGG CC^T[ACGT]AGG EcoO65I 285 GGTGACC G^GT[ACGT]ACC EcoRI 130 GAATTC G^AATTC EcoT22I 1101 ATGCAT ATGCA^T FauI 1383 GCGGG CCCGC(4/6) FbaI 18 TGATCA T^GATCA HaeII 532 AGCGCT [GA]GCGC^[CT] HhaI 533 GCGC GCG^C Hin1I 1183 GACGTC G[GA]^CG[CT]C HinP1I 533 GCGC G^CGC MaeII 1184 ACGT A^CGT MfeI 46 CAATTG C^AATTG MflI 1305 AGATCT [GA]^GATC[CT] MunI 46 CAATTG C^AATTG NsiI 1101 ATGCAT ATGCA^T NspI 737 ACATGT [GA]CATG^[CT] PflFI 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 640 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1428 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 750 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 992 CTGCAG CTGCA^G SapI 216 GAAGAGC GCTCTTC(1/4) ScaI 1373 AGTACT AGT^ACT TaqI 762 TCGA T^CGA Tth111I 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 762 TCGA T^CGA Van91I 640 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1029 TCTAGA T^CTAGA XhoII 1305 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 897 GATCC GGATC(4/5) 120 GGATC AvaII 510 GGACC G^G[AT]CC 1429 GGTCC BglI 560 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 927 GCCTAGAGGGC BpmI 40 CTGGAG CTGGAG(16/14) 1369 CTGGAG BsaBI 16 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 897 GATCCAAATC BsmAI 1392 GAGAC GTCTC(1/5) 1422 GAGAC DdeI 616 CTAAG C^T[ACGT]AG 1390 CTGAG DraI 571 TTTAAA TTT^AAA 1206 TTTAAA DraII 1385 GGGCCCT [GA]G^G[ACGT]CC[CT] 1428 AGGTCCT EarI 216 GAAGAG CTCTTC(1/4) 1078 GAAGAG EcoNI 351 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 615 CCTAAGGCAGG EcoO109I 1385 GGGCCCT [GA]G^G[ACGT]CC[CT] 1428 AGGTCCT EcoT14I 479 CCAAGG C^C[AT][AT]GG 1326 CCTTGG Ksp632I 216 GAAGAG CTCTTC(1/4) 1078 GAAGAG MaeIII 56 GTCAC ^GT[ACGT]AC 286 GTGAC MamI 16 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 897 GATCCAAATC NlaIV 510 GGACCC GG[ACGT]^[ACGT]CC 1385 GGGCCC PleI 113 GAGTC GAGTC(4/5) 279 GAGTC SfaNI 861 GCATC GCATC(5/9) 1231 GCATC SfcI 399 CTATAG C^T[GA][CT]AG 992 CTGCAG SmlI 811 CTTGAG C^T[CT][GA]AG 1317 CTTGAG StuI 482 AGGCCT AGG^CCT 827 AGGCCT StyI 479 CCAAGG C^C[AT][AT]GG 1326 CCTTGG TfiI 526 GAATC G^A[AT]TC 988 GAATC Tsp45I 56 GTCAC ^GT[GC]AC 286 GTGAC XcmI 143 CCAAATACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1010 CCACATTTGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI