Restriction map for
pF1KB4606
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# NO CUT ENZYMES ( 181 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanII BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HapII HincII HindII HindIII
HpaI HpaII KpnI Ksp632I KspI
MaeIII MamI MboI MflI MluI
MroI MseI MspI MvnI NaeI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I TseI Tsp45I Tth111I
Van91I XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 37 GACGTC GACGT^C
AccI 3 GTCTAC GT^[AC][GT]AC
AciI 346 GCGG CCGC(-3/-1)
AluI 93 AGCT AG^CT
AlwNI 25 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 207 GAGCAC G[AT]GC[AT]^C
BalI 194 TGGCCA TGG^CCA
BanI 77 GGCGCC G^G[CT][GA]CC
BbeI 77 GGCGCC GGCGC^C
BfaI 307 CTAG C^TAG
BlpI 94 GCTGAGC GC^T[ACGT]AGC
BmyI 207 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 207 GAGCAC G[AT]GC[AT]^C
BsiYI 221 CCAAGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 221 CCAAGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 225 GAGAC GTCTC(1/5)
BsmBI 225 GAGACG CGTCTC(1/5)
BsmFI 363 GTCCC GGGAC(10/14)
Bsp1286I 207 GAGCAC G[AGT]GC[ACT]^C
BssKI 201 CCTGG ^CC[ACGT]GG
BstNI 201 CCTGG CC^[AT]GG
CelII 94 GCTGAGC GC^T[ACGT]AGC
CfoI 78 GCGC GCG^C
Cfr13I 105 GGCCC G^G[ACGT]CC
DraII 104 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 194 TGGCCA [CT]^GGCC[GA]
EcoO109I 104 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 201 CCTGG ^CC[AT]GG
Fnu4HI 346 GCGGC GC^[ACGT]GC
HaeII 77 GGCGCC [GA]GCGC^[CT]
HgaI 27 GACGC GACGC(5/10)
HhaI 78 GCGC GCG^C
HinP1I 78 GCGC G^CGC
ItaI 346 GCGGC GC^[ACGT]GC
KasI 77 GGCGCC G^GCGCC
MaeI 307 CTAG C^TAG
MaeII 38 ACGT A^CGT
MfeI 72 CAATTG C^AATTG
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MspA1I 92 CAGCTG C[AC]G^C[GT]G
MunI 72 CAATTG C^AATTG
MvaI 201 CCTGG CC^[AT]GG
MwoI 106 GCCCTCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 77 GGCGCC GG^CGCC
NlaIV 77 GGCGCC GG[ACGT]^[ACGT]CC
PvuII 92 CAGCTG CAG^CTG
Sau96I 105 GGCCC G^G[ACGT]CC
ScrFI 201 CCTGG CC^[ACGT]GG
SfaNI 124 GATGC GCATC(5/9)
SfoI 77 GGCGCC GGC^GCC
TaqI 316 TCGA T^CGA
TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 316 TCGA T^CGA
XbaI 306 TCTAGA T^CTAGA
XcmI 330 CCATGAGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AcyI 37 GACGTC G[GA]^CG[CT]C
77 GGCGCC
BbsI 34 GAAGAC GAAGAC(2/6)
40 GTCTTC
BpuAI 34 GAAGAC GAAGAC(2/6)
40 GTCTTC
BsaHI 37 GACGTC G[GA]^CG[CT]C
77 GGCGCC
BsrI 272 ACTGG ACTGG(1/-1)
255 CCAGT
DdeI 95 CTGAG C^T[ACGT]AG
128 CTGAG
Hin1I 37 GACGTC G[GA]^CG[CT]C
77 GGCGCC
HinfI 303 GAATC G^A[ACGT]TC
313 GAATC
HphI 162 GGTGA GGTGA(8/7)
204 GGTGA
NlaIII 159 CATG CATG^
331 CATG
TfiI 303 GAATC G^A[AT]TC
313 GAATC
Tsp509I 16 AATT ^AATT
73 AATT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AfaI BstF5I FokI HaeIII MboII
MnlI MslI RsaI