Restriction map for
pF1KB5126
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BalI BamHI
BbeI BbrPI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI DpnII DraI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 104 GGTACC G^GTACC
AccI 384 GTCTAC GT^[AC][GT]AC
AccII 101 CGCG CG^CG
AcsI 572 GAATTT [GA]^AATT[CT]
AgeI 107 ACCGGT A^CCGGT
AhdI 376 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 572 GAATTT [GA]^AATT[CT]
Asp718I 104 GGTACC G^GTACC
AspEI 376 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 909 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 104 GGTACC G^G[CT][GA]CC
BbsI 826 GAAGAC GAAGAC(2/6)
BbvCI 664 CCTCAGC CCTCAGC(-5/-2)
BcgI 294 CGATTCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 585 GGATAC GTATCC(6/5)
BpuAI 826 GAAGAC GAAGAC(2/6)
BsaWI 107 ACCGGT [AT]^CCGG[AT]
Bsp1407I 756 TGTACA T^GTACA
BsrBI 919 CCGCTC CCGCTC(-3/-3)
BsrGI 756 TGTACA T^GTACA
BstAPI 350 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 661 CATCC GGATG(2/0)
BstUI 101 CGCG CG^CG
CpoI 109 CGGTCCG CG^G[AT]CCG
DrdI 955 GACCATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 376 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 661 CATCC GGATG(9/13)
HphI 691 GGTGA GGTGA(8/7)
KpnI 104 GGTACC GGTAC^C
MaeII 974 ACGT A^CGT
MseI 182 TTAA T^TAA
MvnI 101 CGCG CG^CG
NaeI 163 GCCGGC GCC^GGC
NgoMIV 163 GCCGGC G^CCGGC
PflFI 909 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 997 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 107 ACCGGT A^CCGGT
PstI 621 CTGCAG CTGCA^G
PvuII 752 CAGCTG CAG^CTG
RsrII 109 CGGTCCG CG^G[AT]CCG
SmaI 41 CCCGGG CCC^GGG
SspBI 756 TGTACA T^GTACA
Tru9I 182 TTAA T^TAA
Tth111I 909 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 997 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 299 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
998 CAGCCCCTG
AvaI 41 CCCGGG C^[CT]CG[GA]G
130 CTCGGG
BlpI 463 GCTCAGC GC^T[ACGT]AGC
749 GCTCAGC
BpmI 138 CTCCAG CTGGAG(16/14)
242 CTGGAG
Bpu1102I 463 GCTCAGC GC^T[ACGT]AGC
749 GCTCAGC
BseRI 127 CTCCTC GAGGAG(10/8)
527 CTCCTC
BsoBI 41 CCCGGG C^[CT]CG[GA]G
130 CTCGGG
BspMI 1008 ACCTGC ACCTGC(4/8)
468 GCAGGT
BsrFI 107 ACCGGT [GA]^CCGG[CT]
163 GCCGGC
BsrI 775 ACTGG ACTGG(1/-1)
765 CCAGT
CelII 463 GCTCAGC GC^T[ACGT]AGC
749 GCTCAGC
Cfr10I 107 ACCGGT [GA]^CCGG[CT]
163 GCCGGC
DraIII 794 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
973 CACGTTGTG
HaeII 120 GGCGCT [GA]GCGC^[CT]
152 GGCGCT
MboII 826 GAAGA GAAGA(8/7)
54 TCTTC
MspA1I 591 CCGCTG C[AC]G^C[GT]G
752 CAGCTG
PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT]
455 GGGACCT
SfcI 386 CTACAG C^T[GA][CT]AG
621 CTGCAG
SmlI 399 CTTGAG C^T[CT][GA]AG
852 CTCAAG
TfiI 51 GAATC G^A[AT]TC
295 GATTC
Tsp45I 508 GTCAC ^GT[GC]AC
772 GTCAC
Tsp509I 311 AATT ^AATT
573 AATT
XcmI 231 CCAAACGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
765 CCAGTGTGTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeIII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI SacI Sau96I ScrFI SfaNI
TseI TspRI