Restriction map for
pF1KB4945
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MamI MfeI MluI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 656 TCCGGA T^CCGGA
AcsI 171 GAATTC [GA]^AATT[CT]
AcyI 679 GACGCC G[GA]^CG[CT]C
AflIII 329 ACGTGT A^C[GA][CT]GT
AlwNI 295 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 204 CCTGAGG CC^T[ACGT]AGG
ApaI 534 GGGCCC GGGCC^C
ApoI 171 GAATTC [GA]^AATT[CT]
BalI 272 TGGCCA TGG^CCA
BanI 420 GGTGCC G^G[CT][GA]CC
BbsI 771 GAAGAC GAAGAC(2/6)
BpuAI 771 GAAGAC GAAGAC(2/6)
BsaHI 679 GACGCC G[GA]^CG[CT]C
BsaWI 656 TCCGGA [AT]^CCGG[AT]
BseAI 656 TCCGGA T^CCGGA
BspEI 656 TCCGGA T^CCGGA
BstYI 482 AGATCC [GA]^GATC[CT]
Bsu36I 204 CCTGAGG CC^T[ACGT]AGG
DrdI 541 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 204 CCTGAGG CC^T[ACGT]AGG
EcoRI 171 GAATTC G^AATTC
EcoT14I 275 CCATGG C^C[AT][AT]GG
Hin1I 679 GACGCC G[GA]^CG[CT]C
KspI 45 CCGCGG CCGC^GG
MflI 482 AGATCC [GA]^GATC[CT]
MluNI 272 TGGCCA TGG^CCA
MroI 656 TCCGGA T^CCGGA
MscI 272 TGGCCA TGG^CCA
NcoI 275 CCATGG C^CATGG
NspI 518 ACATGC [GA]CATG^[CT]
PleI 609 GAGTC GAGTC(4/5)
PvuII 862 CAGCTG CAG^CTG
SacI 181 GAGCTC GAGCT^C
SacII 45 CCGCGG CCGC^GG
SfaNI 653 GCATC GCATC(5/9)
SmlI 754 CTCAAG C^T[CT][GA]AG
StuI 201 AGGCCT AGG^CCT
StyI 275 CCATGG C^C[AT][AT]GG
TfiI 624 GAATC G^A[AT]TC
Tsp45I 350 GTGAC ^GT[GC]AC
Tsp509I 172 AATT ^AATT
XhoII 482 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 403 GTCTAC GT^[AC][GT]AC
780 GTCTAC
AccII 46 CGCG CG^CG
108 CGCG
AlwI 337 GATCC GGATC(4/5)
483 GATCC
AspI 679 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
774 GACCGTGTC
BanII 181 GAGCTC G[GA]GC[CT]^C
534 GGGCCC
BpmI 184 CTCCAG CTGGAG(16/14)
865 CTGGAG
BspMI 566 ACCTGC ACCTGC(4/8)
84 GCAGGT
BstUI 46 CGCG CG^CG
108 CGCG
Cac8I 426 GCTTGC GC[ACGT]^[ACGT]GC
631 GCTGGC
DdeI 205 CTGAG C^T[ACGT]AG
469 CTGAG
HgaI 106 GACGC GACGC(5/10)
679 GACGC
HinfI 609 GAGTC G^A[ACGT]TC
624 GAATC
MaeIII 350 GTGAC ^GT[ACGT]AC
465 GTTAC
MboII 480 GAAGA GAAGA(8/7)
771 GAAGA
MslI 276 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
654 CATCCGGATG
MvnI 46 CGCG CG^CG
108 CGCG
PflFI 679 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
774 GACCGTGTC
PstI 82 CTGCAG CTGCA^G
799 CTGCAG
SfcI 82 CTGCAG C^T[GA][CT]AG
799 CTGCAG
TaqI 373 TCGA T^CGA
899 TCGA
Tth111I 679 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
774 GACCGTGTC
TthHB8I 373 TCGA T^CGA
899 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BcnI BmyI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cfr13I DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI TseI TspRI