##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TseI        Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AciI          570  CCGC                 CCGC(-3/-1)
AfaI          585  GTAC                 GT^AC
AhdI          152  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          243  AGCT                 AG^CT
AspEI         152  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         538  GTGCTC               G[AT]GC[AT]^C
BmyI          538  GTGCTC               G[AGT]GC[ACT]^C
BsgI          305  GTGCAG               GTGCAG(16/14)
BsiHKAI       538  GTGCTC               G[AT]GC[AT]^C
BsiYI         570  CCGCTGGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          570  CCGCTGGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         160  GTCCC                GGGAC(10/14)
BsmI          504  GAATGC               GAATGC(1/-1)
Bsp1286I      538  GTGCTC               G[AGT]GC[ACT]^C
Cac8I         199  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI          516  CTTAG                C^T[ACGT]AG
Eam1105I      152  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MaeII         208  ACGT                 A^CGT
MaeIII        554  GTTAC                ^GT[ACGT]AC
MnlI          435  GAGG                 CCTC(7/6)
MseI          133  TTAA                 T^TAA
MspA1I        570  CCGCTG               C[AC]G^C[GT]G
PleI          328  GAGTC                GAGTC(4/5)
RsaI          585  GTAC                 GT^AC
SfcI           46  CTACAG               C^T[GA][CT]AG
Tru9I         133  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BsrI           73  ACTGG                ACTGG(1/-1)
               81  ACTGG               
BstF5I        172  CATCC                GGATG(2/0)
              575  GGATG               
DpnII         223  GATC                 ^GATC
              405  GATC                
EarI          248  GAAGAG               CTCTTC(1/4)
              430  GAAGAG              
FokI          172  CATCC                GGATG(9/13)
              575  GGATG               
Ksp632I       248  GAAGAG               CTCTTC(1/4)
              430  GAAGAG              
MboI          223  GATC                 ^GATC
              405  GATC                
NdeII         223  GATC                 ^GATC
              405  GATC                
Sau3AI        223  GATC                 ^GATC
              405  GATC                
TaqI          246  TCGA                 T^CGA
              407  TCGA                
TfiI          346  GATTC                G^A[AT]TC
              390  GATTC               
TthHB8I       246  TCGA                 T^CGA
              407  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AflIII      BspLU11I    BsrDI       HinfI       MboII       
NlaIII      NspI        TspRI