Restriction map for
pF1KB4828
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccIII AciI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MseI MspA1I NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StyI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 441 GACGTC GACGT^C
AccI 61 GTCTAC GT^[AC][GT]AC
AccII 359 CGCG CG^CG
AcyI 441 GACGTC G[GA]^CG[CT]C
AfaI 536 GTAC GT^AC
AflIII 499 ACATGT A^C[GA][CT]GT
Alw44I 316 GTGCAC G^TGCAC
ApaLI 316 GTGCAC G^TGCAC
Asp700I 859 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 512 GGTCC G^G[AT]CC
BalI 611 TGGCCA TGG^CCA
BbvCI 541 GCTGAGG CCTCAGC(-5/-2)
BclI 278 TGATCA T^GATCA
BglII 20 AGATCT A^GATCT
BpmI 851 CTGGAG CTGGAG(16/14)
BsaHI 441 GACGTC G[GA]^CG[CT]C
BsaI 397 GAGACC GGTCTC(1/5)
BsaJI 38 CCCAGG C^C[ACGT][ACGT]GG
BseRI 544 GAGGAG GAGGAG(10/8)
BsgI 294 CTGCAC GTGCAG(16/14)
BsmFI 117 GGGAC GGGAC(10/14)
BspHI 125 TCATGA T^CATGA
BspLU11I 499 ACATGT A^CATGT
BsrDI 489 CATTGC GCAATG(2/0)
BsrI 850 ACTGG ACTGG(1/-1)
BstAPI 239 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 359 CGCG CG^CG
DraIII 310 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 611 TGGCCA [CT]^GGCC[GA]
FbaI 278 TGATCA T^GATCA
Hin1I 441 GACGTC G[GA]^CG[CT]C
MaeIII 475 GTGAC ^GT[ACGT]AC
MfeI 670 CAATTG C^AATTG
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
MunI 670 CAATTG C^AATTG
MvnI 359 CGCG CG^CG
NlaIV 183 GGCTCC GG[ACGT]^[ACGT]CC
RcaI 125 TCATGA T^CATGA
RsaI 536 GTAC GT^AC
SmlI 685 CTCAAG C^T[CT][GA]AG
SphI 5 GCATGC GCATG^C
SspI 742 AATATT AAT^ATT
Tsp45I 475 GTGAC ^GT[GC]AC
XmnI 859 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 242 GGATC GGATC(4/5)
341 GGATC
AlwNI 240 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
836 CAGAGCCTG
BbsI 108 GAAGAC GAAGAC(2/6)
844 GAAGAC
BfaI 56 CTAG C^TAG
577 CTAG
BpuAI 108 GAAGAC GAAGAC(2/6)
844 GAAGAC
BsaWI 186 TCCGGT [AT]^CCGG[AT]
625 ACCGGA
BsmAI 397 GAGAC GTCTC(1/5)
681 GAGAC
BstYI 20 AGATCT [GA]^GATC[CT]
242 GGATCT
CfoI 360 GCGC GCG^C
453 GCGC
Cfr13I 512 GGTCC G^G[ACGT]CC
584 GGCCC
EarI 822 CTCTTC CTCTTC(1/4)
678 GAAGAG
HhaI 360 GCGC GCG^C
453 GCGC
HinP1I 360 GCGC G^CGC
453 GCGC
HphI 281 TCACC GGTGA(8/7)
623 TCACC
Ksp632I 822 CTCTTC CTCTTC(1/4)
678 GAAGAG
MaeI 56 CTAG C^TAG
577 CTAG
MflI 20 AGATCT [GA]^GATC[CT]
242 GGATCT
MslI 498 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
790 CATGGATATG
NspI 5 GCATGC [GA]CATG^[CT]
499 ACATGT
PstI 237 CTGCAG CTGCA^G
331 CTGCAG
SacI 348 GAGCTC GAGCT^C
370 GAGCTC
Sau96I 512 GGTCC G^G[ACGT]CC
584 GGCCC
StuI 323 AGGCCT AGG^CCT
689 AGGCCT
TfiI 572 GAATC G^A[AT]TC
800 GAATC
Tsp509I 599 AATT ^AATT
671 AATT
XcmI 412 CCACTGAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
494 CCATCACATGTGTGG
XhoII 20 AGATCT [GA]^GATC[CT]
242 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AspHI BanII BbvI BmyI
BsiHKAI Bsp1286I BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
PleI Sau3AI ScrFI SfaNI SfcI
TseI TspRI