Restriction map for
pF1KB4722
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 403 CCGC CCGC(-3/-1)
AcsI 649 AAATTT [GA]^AATT[CT]
AcyI 22 GACGCC G[GA]^CG[CT]C
AfaI 104 GTAC GT^AC
AlwI 427 GGATC GGATC(4/5)
ApoI 649 AAATTT [GA]^AATT[CT]
AspI 483 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 423 CTCGGG C^[CT]CG[GA]G
AvaII 536 GGTCC G^G[AT]CC
BanI 728 GGTGCC G^G[CT][GA]CC
BfaI 166 CTAG C^TAG
BglII 337 AGATCT A^GATCT
BmyI 367 GGGCAC G[AGT]GC[ACT]^C
BsaHI 22 GACGCC G[GA]^CG[CT]C
BseRI 45 CTCCTC GAGGAG(10/8)
BsgI 868 GTGCAG GTGCAG(16/14)
BsmBI 20 GAGACG CGTCTC(1/5)
BsmFI 114 GGGAC GGGAC(10/14)
BsoBI 423 CTCGGG C^[CT]CG[GA]G
Bsp1286I 367 GGGCAC G[AGT]GC[ACT]^C
BspMI 725 GCAGGT ACCTGC(4/8)
Cac8I 165 GCTAGC GC[ACGT]^[ACGT]GC
DraI 603 TTTAAA TTT^AAA
DraII 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 53 CGGCCA [CT]^GGCC[GA]
EcoO109I 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 8 CCAAGG C^C[AT][AT]GG
HgaI 22 GACGC GACGC(5/10)
Hin1I 22 GACGCC G[GA]^CG[CT]C
HphI 459 GGTGA GGTGA(8/7)
MaeI 166 CTAG C^TAG
MboII 175 TCTTC GAAGA(8/7)
NdeI 209 CATATG CA^TATG
NheI 165 GCTAGC G^CTAGC
PflFI 483 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 13 GAGTC GAGTC(4/5)
PpuMI 535 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 104 GTAC GT^AC
SexAI 830 ACCTGGT A^CC[AT]GGT
SmlI 478 CTCAAG C^T[CT][GA]AG
SspI 764 AATATT AAT^ATT
StyI 8 CCAAGG C^C[AT][AT]GG
Tth111I 483 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 26 CCAAAGCACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 556 AGCT AG^CT
909 AGCT
AlwNI 506 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
788 CAGTTACTG
BsaJI 8 CCAAGG C^C[ACGT][ACGT]GG
821 CCGAGG
BssKI 269 CCTGG ^CC[ACGT]GG
831 CCTGG
BstNI 269 CCTGG CC^[AT]GG
831 CCTGG
BstYI 337 AGATCT [GA]^GATC[CT]
427 GGATCT
Cfr13I 39 GGCCC G^G[ACGT]CC
536 GGTCC
DdeI 303 CTCAG C^T[ACGT]AG
903 CTCAG
EcoRII 269 CCTGG ^CC[AT]GG
831 CCTGG
MflI 337 AGATCT [GA]^GATC[CT]
427 GGATCT
MslI 751 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
886 CATACAGATG
MvaI 269 CCTGG CC^[AT]GG
831 CCTGG
Sau96I 39 GGCCC G^G[ACGT]CC
536 GGTCC
ScrFI 269 CCTGG CC^[ACGT]GG
831 CCTGG
SfcI 570 CTGTAG C^T[GA][CT]AG
920 CTACAG
TfiI 280 GATTC G^A[AT]TC
575 GAATC
XhoII 337 AGATCT [GA]^GATC[CT]
427 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
BbvI BsmAI BsrI BstF5I DpnII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MnlI MseI MwoI
NdeII NlaIV Sau3AI SfaNI Tru9I
TseI Tsp509I TspRI