Restriction map for
pF1KB4671
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 700 GAATTT [GA]^AATT[CT]
AflIII 430 ACGTGT A^C[GA][CT]GT
ApoI 700 GAATTT [GA]^AATT[CT]
AspHI 39 GTGCTC G[AT]GC[AT]^C
BalI 282 TGGCCA TGG^CCA
BbvCI 487 CCTCAGC CCTCAGC(-5/-2)
BglII 576 AGATCT A^GATCT
BmyI 39 GTGCTC G[AGT]GC[ACT]^C
BpmI 342 CTCCAG CTGGAG(16/14)
BsaAI 429 TACGTG [CT]AC^GT[GA]
BsaJI 25 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 39 GTGCTC G[AT]GC[AT]^C
BsmFI 347 GTCCC GGGAC(10/14)
Bsp1286I 39 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 433 TGTACA T^GTACA
BsrGI 433 TGTACA T^GTACA
BssSI 514 CTCGTG CACGAG(-5/-1)
BstXI 878 CCAAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 576 AGATCT [GA]^GATC[CT]
CfoI 99 GCGC GCG^C
Cfr13I 850 GGCCC G^G[ACGT]CC
DraI 274 TTTAAA TTT^AAA
EaeI 282 TGGCCA [CT]^GGCC[GA]
EarI 234 GAAGAG CTCTTC(1/4)
HgaI 70 GCGTC GACGC(5/10)
HhaI 99 GCGC GCG^C
HinP1I 99 GCGC G^CGC
HphI 223 TCACC GGTGA(8/7)
Ksp632I 234 GAAGAG CTCTTC(1/4)
MaeII 430 ACGT A^CGT
MflI 576 AGATCT [GA]^GATC[CT]
MluNI 282 TGGCCA TGG^CCA
MscI 282 TGGCCA TGG^CCA
NlaIV 49 GGAGCC GG[ACGT]^[ACGT]CC
SapI 234 GAAGAGC GCTCTTC(1/4)
Sau96I 850 GGCCC G^G[ACGT]CC
SfaNI 499 GATGC GCATC(5/9)
SfcI 639 CTACAG C^T[GA][CT]AG
SmlI 766 CTCAAG C^T[CT][GA]AG
SphI 718 GCATGC GCATG^C
SspBI 433 TGTACA T^GTACA
TaqI 174 TCGA T^CGA
TfiI 21 GATTC G^A[AT]TC
TthHB8I 174 TCGA T^CGA
XbaI 505 TCTAGA T^CTAGA
XhoII 576 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 434 GTAC GT^AC
901 GTAC
BclI 144 TGATCA T^GATCA
258 TGATCA
BfaI 358 CTAG C^TAG
506 CTAG
BseRI 580 CTCCTC GAGGAG(10/8)
530 GAGGAG
BsmAI 829 GAGAC GTCTC(1/5)
313 GTCTC
BssKI 25 CCTGG ^CC[ACGT]GG
307 CCAGG
BstNI 25 CCTGG CC^[AT]GG
307 CCAGG
EcoRII 25 CCTGG ^CC[AT]GG
307 CCAGG
FbaI 144 TGATCA T^GATCA
258 TGATCA
MaeI 358 CTAG C^TAG
506 CTAG
MvaI 25 CCTGG CC^[AT]GG
307 CCAGG
MwoI 4 GCCTGTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
92 GCACTTTGCGC
NspI 718 GCATGC [GA]CATG^[CT]
729 GCATGT
RsaI 434 GTAC GT^AC
901 GTAC
ScrFI 25 CCTGG CC^[ACGT]GG
307 CCAGG
Tsp45I 72 GTCAC ^GT[GC]AC
222 GTCAC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsrI BstF5I Cac8I DdeI DpnII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII PleI Sau3AI Tru9I
TseI Tsp509I TspRI