Restriction map for
pF1KE9636
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BbrPI
BclI BfrI BlnI BsiEI BsiWI
BsmBI BsmI BspDI BspLU11I BssHII
BstBI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoT22I FbaI
FseI HindIII HpaI KpnI MfeI
MluI MunI NheI NotI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AhdI 2852 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1263 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2852 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3405 TGCGCA TGC^GCA
BalI 749 TGGCCA TGG^CCA
BamHI 4295 GGATCC G^GATCC
BciVI 3476 GTATCC GTATCC(6/5)
BsaBI 2004 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3371 TGTACA T^GTACA
BsrGI 3371 TGTACA T^GTACA
BssSI 2657 CTCGTG CACGAG(-5/-1)
Bst1107I 2247 GTATAC GTA^TAC
BstZ17I 2247 GTATAC GTA^TAC
DraI 1207 TTTAAA TTT^AAA
DraIII 2518 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2173 GACGGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2852 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1474 GATATC GAT^ATC
FspI 3405 TGCGCA TGC^GCA
KspI 146 CCGCGG CCGC^GG
MamI 2004 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
NruI 92 TCGCGA TCG^CGA
NspI 1226 ACATGC [GA]CATG^[CT]
PshAI 3604 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 146 CCGCGG CCGC^GG
SexAI 1594 ACCTGGT A^CC[AT]GGT
SspBI 3371 TGTACA T^GTACA
SspI 442 AATATT AAT^ATT
XmnI 1263 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 654 TCCGGA T^CCGGA
1904 TCCGGA
AflIII 529 ACACGT A^C[GA][CT]GT
2134 ACGTGT
BbeI 1874 GGCGCC GGCGC^C
3749 GGCGCC
BcgI 112 GCAGAGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3483 GCAGCTGCGTCG
BglII 707 AGATCT A^GATCT
4113 AGATCT
BlpI 2071 GCTGAGC GC^T[ACGT]AGC
3922 GCTGAGC
Bpu1102I 2071 GCTGAGC GC^T[ACGT]AGC
3922 GCTGAGC
BsaAI 1504 TACGTG [CT]AC^GT[GA]
2133 TACGTG
BsaI 74 GAGACC GGTCTC(1/5)
3763 GAGACC
BseAI 654 TCCGGA T^CCGGA
1904 TCCGGA
BspEI 654 TCCGGA T^CCGGA
1904 TCCGGA
BspHI 1706 TCATGA T^CATGA
4202 TCATGA
BsrBI 2026 GAGCGG CCGCTC(-3/-3)
2404 GAGCGG
BsrDI 2931 CATTGC GCAATG(2/0)
947 GCAATG
BstEII 3121 GGTGACC G^GT[ACGT]ACC
4227 GGTGACC
BstPI 3121 GGTGACC G^GT[ACGT]ACC
4227 GGTGACC
CelII 2071 GCTGAGC GC^T[ACGT]AGC
3922 GCTGAGC
EcoO65I 3121 GGTGACC G^GT[ACGT]ACC
4227 GGTGACC
KasI 1874 GGCGCC G^GCGCC
3749 GGCGCC
MroI 654 TCCGGA T^CCGGA
1904 TCCGGA
NarI 1874 GGCGCC GG^CGCC
3749 GGCGCC
NcoI 1731 CCATGG C^CATGG
3624 CCATGG
PaeR7I 989 CTCGAG C^TCGAG
4222 CTCGAG
RcaI 1706 TCATGA T^CATGA
4202 TCATGA
SapI 2023 GAAGAGC GCTCTTC(1/4)
4483 GAAGAGC
SfoI 1874 GGCGCC GGC^GCC
3749 GGCGCC
StuI 2639 AGGCCT AGG^CCT
3248 AGGCCT
XhoI 989 CTCGAG C^TCGAG
4222 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI