Restriction map for
pF1KB4569
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1351 GTCTAC GT^[AC][GT]AC
AccII 1367 CGCG CG^CG
AlwNI 1482 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1345 CCTGAGG CC^T[ACGT]AGG
ApaI 1370 GGGCCC GGGCC^C
BamHI 524 GGATCC G^GATCC
BbrPI 124 CACGTG CAC^GTG
BbvCI 309 CCTCAGC CCTCAGC(-5/-2)
BfaI 133 CTAG C^TAG
BglI 542 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 124 CACGTG [CT]AC^GT[GA]
BsaBI 300 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1531 GAGACC GGTCTC(1/5)
BsgI 1607 GTGCAG GTGCAG(16/14)
BsiEI 1590 CGGCCG CG[GA][CT]^CG
BsmAI 1531 GAGAC GTCTC(1/5)
BsrBI 739 CCGCTC CCGCTC(-3/-3)
BsrFI 33 GCCGGT [GA]^CCGG[CT]
BstUI 1367 CGCG CG^CG
BstXI 863 CCATGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 524 GGATCC [GA]^GATC[CT]
Bsu36I 1345 CCTGAGG CC^T[ACGT]AGG
Cfr10I 33 GCCGGT [GA]^CCGG[CT]
EaeI 1590 CGGCCG [CT]^GGCC[GA]
EagI 1590 CGGCCG C^GGCCG
EclXI 1590 CGGCCG C^GGCCG
Eco52I 1590 CGGCCG C^GGCCG
Eco81I 1345 CCTGAGG CC^T[ACGT]AGG
EcoT22I 980 ATGCAT ATGCA^T
HgaI 582 GACGC GACGC(5/10)
MaeI 133 CTAG C^TAG
MaeII 125 ACGT A^CGT
MamI 300 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 524 GGATCC [GA]^GATC[CT]
MseI 832 TTAA T^TAA
MslI 294 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1367 CGCG CG^CG
NdeI 1331 CATATG CA^TATG
NsiI 980 ATGCAT ATGCA^T
PmaCI 124 CACGTG CAC^GTG
PmlI 124 CACGTG CAC^GTG
PpuMI 917 GGGACCT [GA]G^G[AT]CC[CT]
SacI 857 GAGCTC GAGCT^C
SexAI 1618 ACCAGGT A^CC[AT]GGT
TfiI 1450 GAATC G^A[AT]TC
Tru9I 832 TTAA T^TAA
XhoII 524 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 43 AAATTC [GA]^AATT[CT]
241 AAATTT
ApoI 43 AAATTC [GA]^AATT[CT]
241 AAATTT
BanI 321 GGCACC G^G[CT][GA]CC
1384 GGCACC
BanII 857 GAGCTC G[GA]GC[CT]^C
1370 GGGCCC
BbsI 898 GAAGAC GAAGAC(2/6)
1200 GAAGAC
BcnI 537 CCGGG CC^[GC]GG
1566 CCGGG
BpuAI 898 GAAGAC GAAGAC(2/6)
1200 GAAGAC
BsmI 1598 GAATGC GAATGC(1/-1)
982 GCATTC
CfoI 508 GCGC GCG^C
1194 GCGC
EarI 1021 CTCTTC CTCTTC(1/4)
1288 CTCTTC
EcoNI 830 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1101 CCTGAGTGAGG
FauI 738 CCCGC CCCGC(4/6)
1368 GCGGG
HhaI 508 GCGC GCG^C
1194 GCGC
HinP1I 508 GCGC G^CGC
1194 GCGC
Ksp632I 1021 CTCTTC CTCTTC(1/4)
1288 CTCTTC
NciI 537 CCGGG CC^[GC]GG
1566 CCGGG
NcoI 293 CCATGG C^CATGG
1205 CCATGG
PflMI 410 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCAGGAGATGG
TaqI 668 TCGA T^CGA
1624 TCGA
Tsp45I 580 GTGAC ^GT[GC]AC
1165 GTGAC
Tsp509I 44 AATT ^AATT
242 AATT
TthHB8I 668 TCGA T^CGA
1624 TCGA
Van91I 410 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI