Restriction map for
pF1KB4553
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MboI MfeI
MflI MluI MroI MslI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 107 CGCG CG^CG
AfaI 112 GTAC GT^AC
ApaI 24 GGGCCC GGGCC^C
AvaII 315 GGTCC G^G[AT]CC
AviII 380 TGCGCA TGC^GCA
BbvI 167 GCTGC GCAGC(8/12)
BlpI 51 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
BseRI 161 CTCCTC GAGGAG(10/8)
BsiYI 318 CCTCATTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 318 CCTCATTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 417 CATTGC GCAATG(2/0)
BstAPI 264 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 107 CGCG CG^CG
CelII 51 GCTGAGC GC^T[ACGT]AGC
EcoNI 318 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 404 CCAAGG C^C[AT][AT]GG
Fnu4HI 167 GCTGC GC^[ACGT]GC
FspI 380 TGCGCA TGC^GCA
HaeII 249 GGCGCT [GA]GCGC^[CT]
HgaI 105 GACGC GACGC(5/10)
HphI 32 TCACC GGTGA(8/7)
ItaI 167 GCTGC GC^[ACGT]GC
MaeII 311 ACGT A^CGT
MseI 356 TTAA T^TAA
MvnI 107 CGCG CG^CG
NspI 269 GCATGC [GA]CATG^[CT]
RsaI 112 GTAC GT^AC
SfaNI 170 GCATC GCATC(5/9)
SphI 269 GCATGC GCATG^C
StyI 404 CCAAGG C^C[AT][AT]GG
TaqI 299 TCGA T^CGA
Tru9I 356 TTAA T^TAA
TseI 167 GCTGC G^C[AT]GC
Tsp509I 354 AATT ^AATT
TthHB8I 299 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 108 GCGG CCGC(-3/-1)
387 GCGG
BalI 224 TGGCCA TGG^CCA
413 TGGCCA
BsmFI 234 GGGAC GGGAC(10/14)
346 GGGAC
BssKI 135 CCTGG ^CC[ACGT]GG
274 CCTGG
BstF5I 171 CATCC GGATG(2/0)
366 CATCC
BstNI 135 CCTGG CC^[AT]GG
274 CCTGG
BstXI 305 CCAACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
404 CCAAGGTGCTGG
CfoI 250 GCGC GCG^C
381 GCGC
DraII 23 GGGGCCC [GA]G^G[ACGT]CC[CT]
24 GGGCCCC
EaeI 224 TGGCCA [CT]^GGCC[GA]
413 TGGCCA
EcoO109I 23 GGGGCCC [GA]G^G[ACGT]CC[CT]
24 GGGCCCC
EcoRII 135 CCTGG ^CC[AT]GG
274 CCTGG
FauI 108 GCGGG CCCGC(4/6)
387 GCGGG
FokI 171 CATCC GGATG(9/13)
366 CATCC
HhaI 250 GCGC GCG^C
381 GCGC
HinP1I 250 GCGC G^CGC
381 GCGC
MluNI 224 TGGCCA TGG^CCA
413 TGGCCA
MscI 224 TGGCCA TGG^CCA
413 TGGCCA
MvaI 135 CCTGG CC^[AT]GG
274 CCTGG
NlaIII 175 CATG CATG^
270 CATG
PleI 301 GACTC GAGTC(4/5)
81 GAGTC
ScrFI 135 CCTGG CC^[ACGT]GG
274 CCTGG
TfiI 93 GATTC G^A[AT]TC
296 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BanII BmyI BsaJI Bsp1286I BsrI
Cac8I Cfr13I DdeI HaeIII HinfI
MboII MnlI MwoI NlaIV Sau96I
TspRI