Restriction map for
pF1KB4518
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII Cfr10I ClaI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FauI FseI
HgaI Hin1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 588 CGCG CG^CG
AcsI 736 GAATTC [GA]^AATT[CT]
AhdI 217 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 282 GATCC GGATC(4/5)
AocI 141 CCTCAGG CC^T[ACGT]AGG
ApoI 736 GAATTC [GA]^AATT[CT]
AspEI 217 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 956 GAGCAC G[AT]GC[AT]^C
AviII 71 TGCGCA TGC^GCA
BalI 869 TGGCCA TGG^CCA
BbsI 25 GTCTTC GAAGAC(2/6)
BcgI 597 CGATGTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 242 TGATCA T^GATCA
BglII 542 AGATCT A^GATCT
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaI 560 GAGACC GGTCTC(1/5)
BsgI 328 GTGCAG GTGCAG(16/14)
BsiHKAI 956 GAGCAC G[AT]GC[AT]^C
BsmI 580 GAATGC GAATGC(1/-1)
BspMI 319 ACCTGC ACCTGC(4/8)
BssSI 236 CTCGTG CACGAG(-5/-1)
BstEII 165 GGTAACC G^GT[ACGT]ACC
BstPI 165 GGTAACC G^GT[ACGT]ACC
BstUI 588 CGCG CG^CG
BstYI 542 AGATCT [GA]^GATC[CT]
Bsu36I 141 CCTCAGG CC^T[ACGT]AGG
Cac8I 898 GCCAGC GC[ACGT]^[ACGT]GC
CpoI 116 CGGTCCG CG^G[AT]CCG
DraII 982 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 728 CCATGG C^C[GA][CT]GG
EaeI 869 TGGCCA [CT]^GGCC[GA]
Eam1105I 217 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 49 CTCTTC CTCTTC(1/4)
Eco81I 141 CCTCAGG CC^T[ACGT]AGG
EcoO109I 982 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 165 GGTAACC G^GT[ACGT]ACC
EcoRI 736 GAATTC G^AATTC
EcoT14I 728 CCATGG C^C[AT][AT]GG
FbaI 242 TGATCA T^GATCA
FspI 71 TGCGCA TGC^GCA
HaeII 133 AGCGCC [GA]GCGC^[CT]
HindIII 880 AAGCTT A^AGCTT
HphI 363 TCACC GGTGA(8/7)
Ksp632I 49 CTCTTC CTCTTC(1/4)
MflI 542 AGATCT [GA]^GATC[CT]
MluNI 869 TGGCCA TGG^CCA
MscI 869 TGGCCA TGG^CCA
MslI 411 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 75 CAGCGG C[AC]G^C[GT]G
MvnI 588 CGCG CG^CG
NcoI 728 CCATGG C^CATGG
NspI 809 GCATGT [GA]CATG^[CT]
PflMI 285 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 982 GGGACCC [GA]G^G[AT]CC[CT]
PstI 719 CTGCAG CTGCA^G
RsrII 116 CGGTCCG CG^G[AT]CCG
SapI 48 GCTCTTC GCTCTTC(1/4)
SexAI 760 ACCAGGT A^CC[AT]GGT
SfcI 719 CTGCAG C^T[GA][CT]AG
StyI 728 CCATGG C^C[AT][AT]GG
Tsp509I 737 AATT ^AATT
Van91I 285 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 472 CCAACTGTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 542 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 170 CCCGGG C^[CT]CG[GA]G
356 CCCGGG
BmyI 64 GTGCCC G[AGT]GC[ACT]^C
956 GAGCAC
BseRI 383 CTCCTC GAGGAG(10/8)
402 CTCCTC
BsoBI 170 CCCGGG C^[CT]CG[GA]G
356 CCCGGG
Bsp1286I 64 GTGCCC G[AGT]GC[ACT]^C
956 GAGCAC
BspHI 278 TCATGA T^CATGA
392 TCATGA
MaeII 110 ACGT A^CGT
352 ACGT
NlaIV 982 GGGACC GG[ACGT]^[ACGT]CC
983 GGACCC
PshAI 304 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
488 GACACCTGTC
RcaI 278 TCATGA T^CATGA
392 TCATGA
SmaI 170 CCCGGG CCC^GGG
356 CCCGGG
XmaCI 170 CCCGGG C^CCGGG
356 CCCGGG
XmaI 170 CCCGGG C^CCGGG
356 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstXI CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI