Restriction map for
pF1KB4501
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MluI MroI
MspA1I MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tsp45I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 172 CGCG CG^CG
AciI 171 CCGC CCGC(-3/-1)
AcsI 304 GAATTC [GA]^AATT[CT]
AlwI 374 GGATC GGATC(4/5)
ApoI 304 GAATTC [GA]^AATT[CT]
AspI 336 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 434 CCCGAG C^[CT]CG[GA]G
AvaII 545 GGTCC G^G[AT]CC
BalI 69 TGGCCA TGG^CCA
BbvI 256 GCTGC GCAGC(8/12)
BcgI 100 CGACTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 561 AGATCT A^GATCT
BsaI 357 GGTCTC GGTCTC(1/5)
BsgI 397 GTGCAG GTGCAG(16/14)
BsmFI 570 GTCCC GGGAC(10/14)
BsmI 259 GCATTC GAATGC(1/-1)
BsoBI 434 CCCGAG C^[CT]CG[GA]G
BspMI 455 ACCTGC ACCTGC(4/8)
BstAPI 259 GCATTCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 172 CGCG CG^CG
BstXI 33 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 399 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 173 GCGC GCG^C
DsaI 406 CCACGG C^C[GA][CT]GG
EaeI 69 TGGCCA [CT]^GGCC[GA]
EcoRI 304 GAATTC G^AATTC
EcoT14I 492 CCAAGG C^C[AT][AT]GG
Fnu4HI 256 GCTGC GC^[ACGT]GC
HhaI 173 GCGC GCG^C
HinP1I 173 GCGC G^CGC
HincII 342 GTCAAC GT[CT]^[GA]AC
HindII 342 GTCAAC GT[CT]^[GA]AC
ItaI 256 GCTGC GC^[ACGT]GC
MluNI 69 TGGCCA TGG^CCA
MscI 69 TGGCCA TGG^CCA
MseI 13 TTAA T^TAA
MslI 241 CATAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 172 CGCG CG^CG
MwoI 259 GCATTCCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 336 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 457 CTGCAG CTGCA^G
SbfI 456 CCTGCAGG CCTGCA^GG
SexAI 337 ACCAGGT A^CC[AT]GGT
SfaNI 107 GATGC GCATC(5/9)
SfcI 457 CTGCAG C^T[GA][CT]AG
SmlI 178 CTCAAG C^T[CT][GA]AG
Sse8387I 456 CCTGCAGG CCTGCA^GG
StyI 492 CCAAGG C^C[AT][AT]GG
TfiI 217 GATTC G^A[AT]TC
Tru9I 13 TTAA T^TAA
TseI 256 GCTGC G^C[AT]GC
Tsp509I 305 AATT ^AATT
TspRI 288 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 336 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 502 GTAC GT^AC
555 GTAC
AluI 147 AGCT AG^CT
201 AGCT
AocI 52 CCTCAGG CC^T[ACGT]AGG
540 CCTGAGG
BbsI 276 GAAGAC GAAGAC(2/6)
333 GAAGAC
BpuAI 276 GAAGAC GAAGAC(2/6)
333 GAAGAC
BsmAI 114 GTCTC GTCTC(1/5)
358 GTCTC
BssSI 444 CACGAG CACGAG(-5/-1)
129 CTCGTG
BstYI 374 GGATCT [GA]^GATC[CT]
561 AGATCT
Bsu36I 52 CCTCAGG CC^T[ACGT]AGG
540 CCTGAGG
Cfr13I 366 GGCCC G^G[ACGT]CC
545 GGTCC
DpnII 375 GATC ^GATC
562 GATC
Eco81I 52 CCTCAGG CC^T[ACGT]AGG
540 CCTGAGG
MboI 375 GATC ^GATC
562 GATC
MflI 374 GGATCT [GA]^GATC[CT]
561 AGATCT
NdeII 375 GATC ^GATC
562 GATC
NlaIII 60 CATG CATG^
513 CATG
RsaI 502 GTAC GT^AC
555 GTAC
Sau3AI 375 GATC ^GATC
562 GATC
Sau96I 366 GGCCC G^G[ACGT]CC
545 GGTCC
TaqI 99 TCGA T^CGA
190 TCGA
TthHB8I 99 TCGA T^CGA
190 TCGA
XhoII 374 GGATCT [GA]^GATC[CT]
561 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
BsaJI BseRI BsrI BssKI BstF5I
BstNI DdeI EcoRII FokI HaeIII
HinfI MboII MnlI MvaI NlaIV
PleI ScrFI