Restriction map for
pF1KB4480
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaJI BsaWI BseAI BsiEI
BsiWI BsmFI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstF5I BstPI
BstUI BstXI BstYI CelII Cfr10I
ClaI CpoI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FauI FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 852 GTATAC GT^[AC][GT]AC
AciI 931 GCGG CCGC(-3/-1)
AcyI 341 GGCGCC G[GA]^CG[CT]C
AflII 733 CTTAAG C^TTAAG
AocI 910 CCTAAGG CC^T[ACGT]AGG
AseI 726 ATTAAT AT^TAAT
AsnI 726 ATTAAT AT^TAAT
AspI 658 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 231 GGTCC G^G[AT]CC
BanI 341 GGCGCC G^G[CT][GA]CC
BanII 1040 GAGCTC G[GA]GC[CT]^C
BbeI 341 GGCGCC GGCGC^C
BbrPI 706 CACGTG CAC^GTG
BbsI 842 GTCTTC GAAGAC(2/6)
BbvI 532 GCTGC GCAGC(8/12)
BfrI 733 CTTAAG C^TTAAG
BpuAI 842 GTCTTC GAAGAC(2/6)
BsaAI 706 CACGTG [CT]AC^GT[GA]
BsaHI 341 GGCGCC G[GA]^CG[CT]C
BsiYI 832 CCCAGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 832 CCCAGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 97 GAGACG CGTCTC(1/5)
Bsp1407I 582 TGTACA T^GTACA
BspHI 363 TCATGA T^CATGA
BspMI 410 GCAGGT ACCTGC(4/8)
BsrGI 582 TGTACA T^GTACA
BsrI 800 ACTGG ACTGG(1/-1)
Bst1107I 852 GTATAC GTA^TAC
BstZ17I 852 GTATAC GTA^TAC
Bsu36I 910 CCTAAGG CC^T[ACGT]AGG
DraI 918 TTTAAA TTT^AAA
DraIII 325 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 910 CCTAAGG CC^T[ACGT]AGG
EcoRI 1006 GAATTC G^AATTC
EcoT22I 722 ATGCAT ATGCA^T
Fnu4HI 532 GCTGC GC^[ACGT]GC
HaeII 341 GGCGCC [GA]GCGC^[CT]
HaeIII 902 GGCC GG^CC
HapII 345 CCGG C^CGG
HgaI 99 GACGC GACGC(5/10)
Hin1I 341 GGCGCC G[GA]^CG[CT]C
HpaII 345 CCGG C^CGG
ItaI 532 GCTGC GC^[ACGT]GC
KasI 341 GGCGCC G^GCGCC
MaeII 707 ACGT A^CGT
MspI 345 CCGG C^CGG
MwoI 333 GCTTGACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 341 GGCGCC GG^CGCC
NlaIV 341 GGCGCC GG[ACGT]^[ACGT]CC
NsiI 722 ATGCAT ATGCA^T
PflFI 658 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 132 GAGTC GAGTC(4/5)
PmaCI 706 CACGTG CAC^GTG
PmlI 706 CACGTG CAC^GTG
PshBI 726 ATTAAT AT^TAAT
PstI 533 CTGCAG CTGCA^G
RcaI 363 TCATGA T^CATGA
SacI 1040 GAGCTC GAGCT^C
SapI 179 GCTCTTC GCTCTTC(1/4)
SfaNI 268 GATGC GCATC(5/9)
SfcI 533 CTGCAG C^T[GA][CT]AG
SfoI 341 GGCGCC GGC^GCC
SmlI 733 CTTAAG C^T[CT][GA]AG
SspBI 582 TGTACA T^GTACA
SwaI 917 ATTTAAAT ATTT^AAAT
TseI 532 GCTGC G^C[AT]GC
Tth111I 658 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AfaI 583 GTAC GT^AC
969 GTAC
AspHI 88 GAGCAC G[AT]GC[AT]^C
1040 GAGCTC
BfaI 235 CTAG C^TAG
511 CTAG
BmyI 88 GAGCAC G[AGT]GC[ACT]^C
1040 GAGCTC
BsgI 30 CTGCAC GTGCAG(16/14)
425 GTGCAG
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
1040 GAGCTC
BsmAI 97 GAGAC GTCTC(1/5)
774 GAGAC
BsmI 995 GAATGC GAATGC(1/-1)
446 GCATTC
Bsp1286I 88 GAGCAC G[AGT]GC[ACT]^C
1040 GAGCTC
BssKI 93 CCAGG ^CC[ACGT]GG
310 CCTGG
BstBI 167 TTCGAA TT^CGAA
605 TTCGAA
BstNI 93 CCAGG CC^[AT]GG
310 CCTGG
Cac8I 175 GCTGGC GC[ACGT]^[ACGT]GC
982 GCAAGC
CfoI 3 GCGC GCG^C
342 GCGC
Cfr13I 231 GGTCC G^G[ACGT]CC
902 GGCCC
EarI 180 CTCTTC CTCTTC(1/4)
15 GAAGAG
EcoRII 93 CCAGG ^CC[AT]GG
310 CCTGG
HhaI 3 GCGC GCG^C
342 GCGC
HinP1I 3 GCGC G^CGC
342 GCGC
Ksp632I 180 CTCTTC CTCTTC(1/4)
15 GAAGAG
MaeI 235 CTAG C^TAG
511 CTAG
MslI 886 CACCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
943 CATTATTATG
MvaI 93 CCAGG CC^[AT]GG
310 CCTGG
NspV 167 TTCGAA TT^CGAA
605 TTCGAA
RsaI 583 GTAC GT^AC
969 GTAC
Sau96I 231 GGTCC G^G[ACGT]CC
902 GGCCC
ScrFI 93 CCAGG CC^[ACGT]GG
310 CCTGG
SfuI 167 TTCGAA TT^CGAA
605 TTCGAA
TaqI 168 TCGA T^CGA
606 TCGA
TthHB8I 168 TCGA T^CGA
606 TCGA
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
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AcsI AluI ApoI BseRI DdeI
DpnII HinfI HphI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI TfiI Tru9I Tsp45I Tsp509I
TspRI