Restriction map for
pF1KB4417
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MboII MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Asp700I 259 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 722 CTCGAG C^[CT]CG[GA]G
AviII 345 TGCGCA TGC^GCA
BbvCI 1433 CCTCAGC CCTCAGC(-5/-2)
BsaI 725 GAGACC GGTCTC(1/5)
BsmBI 1318 GAGACG CGTCTC(1/5)
BsmI 263 GCATTC GAATGC(1/-1)
BsoBI 722 CTCGAG C^[CT]CG[GA]G
Bsp1407I 701 TGTACA T^GTACA
BspHI 977 TCATGA T^CATGA
BsrBI 546 GAGCGG CCGCTC(-3/-3)
BsrFI 340 GCCGGT [GA]^CCGG[CT]
BsrGI 701 TGTACA T^GTACA
BstXI 784 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 340 GCCGGT [GA]^CCGG[CT]
EaeI 906 TGGCCG [CT]^GGCC[GA]
EcoNI 759 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 588 CCATGG C^C[AT][AT]GG
FspI 345 TGCGCA TGC^GCA
HaeII 215 AGCGCC [GA]GCGC^[CT]
HincII 1240 GTCAAC GT[CT]^[GA]AC
HindII 1240 GTCAAC GT[CT]^[GA]AC
KspI 1202 CCGCGG CCGC^GG
NcoI 588 CCATGG C^CATGG
NdeI 1229 CATATG CA^TATG
NspI 572 ACATGC [GA]CATG^[CT]
PaeR7I 722 CTCGAG C^TCGAG
PflMI 605 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 672 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 977 TCATGA T^CATGA
SacII 1202 CCGCGG CCGC^GG
SbfI 528 CCTGCAGG CCTGCA^GG
SgrAI 339 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 722 CTCGAG C^T[CT][GA]AG
Sse8387I 528 CCTGCAGG CCTGCA^GG
SspBI 701 TGTACA T^GTACA
StyI 588 CCATGG C^C[AT][AT]GG
TfiI 1223 GAATC G^A[AT]TC
Van91I 605 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 605 CCAAGTTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 722 CTCGAG C^TCGAG
XmnI 259 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1203 CGCG CG^CG
1270 CGCG
AcyI 592 GGCGTC G[GA]^CG[CT]C
1069 GGCGTC
AocI 268 CCTGAGG CC^T[ACGT]AGG
1392 CCTGAGG
ApaI 205 GGGCCC GGGCC^C
508 GGGCCC
BcnI 797 CCCGG CC^[GC]GG
953 CCCGG
BglII 737 AGATCT A^GATCT
839 AGATCT
BsaHI 592 GGCGTC G[GA]^CG[CT]C
1069 GGCGTC
BseRI 43 GAGGAG GAGGAG(10/8)
433 GAGGAG
BspMI 527 ACCTGC ACCTGC(4/8)
986 ACCTGC
BstAPI 531 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1451 GCAACGCCTGC
BstUI 1203 CGCG CG^CG
1270 CGCG
BstYI 737 AGATCT [GA]^GATC[CT]
839 AGATCT
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
1392 CCTGAGG
DpnII 738 GATC ^GATC
840 GATC
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
1392 CCTGAGG
Hin1I 592 GGCGTC G[GA]^CG[CT]C
1069 GGCGTC
MboI 738 GATC ^GATC
840 GATC
MflI 737 AGATCT [GA]^GATC[CT]
839 AGATCT
MvnI 1203 CGCG CG^CG
1270 CGCG
NciI 797 CCCGG CC^[GC]GG
953 CCCGG
NdeII 738 GATC ^GATC
840 GATC
PleI 162 GACTC GAGTC(4/5)
1405 GACTC
PpuMI 113 GGGTCCT [GA]G^G[AT]CC[CT]
437 AGGTCCT
Sau3AI 738 GATC ^GATC
840 GATC
SfaNI 195 GCATC GCATC(5/9)
485 GCATC
StuI 320 AGGCCT AGG^CCT
746 AGGCCT
TaqI 723 TCGA T^CGA
1221 TCGA
TthHB8I 723 TCGA T^CGA
1221 TCGA
XhoII 737 AGATCT [GA]^GATC[CT]
839 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BanII BbvI BglI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MnlI MslI
MspA1I MspI MvaI MwoI NlaIII
NlaIV PstI PvuII RsaI Sau96I
ScrFI SfcI TseI Tsp45I TspRI