Restriction map for
pF1KB4327
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BciVI BfrI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 514 GTCTAC GT^[AC][GT]AC
AccII 382 CGCG CG^CG
AfaI 161 GTAC GT^AC
Aor51HI 118 AGCGCT AGC^GCT
ApaI 414 GGGCCC GGGCC^C
AvaI 1504 CCCGAG C^[CT]CG[GA]G
BalI 1028 TGGCCA TGG^CCA
BamHI 770 GGATCC G^GATCC
BbeI 27 GGCGCC GGCGC^C
BbrPI 1426 CACGTG CAC^GTG
BbsI 1057 GTCTTC GAAGAC(2/6)
BcgI 176 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1215 AGATCT A^GATCT
BlpI 778 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 778 GCTGAGC GC^T[ACGT]AGC
BpuAI 1057 GTCTTC GAAGAC(2/6)
BsaAI 1426 CACGTG [CT]AC^GT[GA]
BsaI 1483 GAGACC GGTCTC(1/5)
BsgI 174 GTGCAG GTGCAG(16/14)
BsmI 1457 GCATTC GAATGC(1/-1)
BsoBI 1504 CCCGAG C^[CT]CG[GA]G
BssSI 171 CTCGTG CACGAG(-5/-1)
BstAPI 1425 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 382 CGCG CG^CG
CelII 778 GCTGAGC GC^T[ACGT]AGC
DrdI 505 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 118 AGCGCT AGC^GCT
HincII 502 GTTGAC GT[CT]^[GA]AC
HindII 502 GTTGAC GT[CT]^[GA]AC
KasI 27 GGCGCC G^GCGCC
MluNI 1028 TGGCCA TGG^CCA
MscI 1028 TGGCCA TGG^CCA
MvnI 382 CGCG CG^CG
NaeI 1535 GCCGGC GCC^GGC
NarI 27 GGCGCC GG^CGCC
NgoMIV 1535 GCCGGC G^CCGGC
PleI 712 GAGTC GAGTC(4/5)
PmaCI 1426 CACGTG CAC^GTG
PmlI 1426 CACGTG CAC^GTG
PpuMI 836 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 161 GTAC GT^AC
SapI 1257 GCTCTTC GCTCTTC(1/4)
SbfI 831 CCTGCAGG CCTGCA^GG
SfiI 819 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 27 GGCGCC GGC^GCC
SmlI 211 CTCAAG C^T[CT][GA]AG
Sse8387I 831 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 802 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGGACCTG
BanI 27 GGCGCC G^G[CT][GA]CC
1036 GGCACC
BclI 656 TGATCA T^GATCA
1346 TGATCA
BfaI 127 CTAG C^TAG
1357 CTAG
BsmAI 1483 GAGAC GTCTC(1/5)
625 GTCTC
BsmFI 221 GGGAC GGGAC(10/14)
1517 GGGAC
BspMI 850 ACCTGC ACCTGC(4/8)
294 GCAGGT
BsrBI 662 CCGCTC CCGCTC(-3/-3)
1377 GAGCGG
BsrI 158 CCAGT ACTGG(1/-1)
264 CCAGT
BstEII 355 GGTTACC G^GT[ACGT]ACC
1068 GGTCACC
BstPI 355 GGTTACC G^GT[ACGT]ACC
1068 GGTCACC
DraIII 1182 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1426 CACGTGGTG
EcoNI 979 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1500 CCTGCCCGAGG
EcoO65I 355 GGTTACC G^GT[ACGT]ACC
1068 GGTCACC
FbaI 656 TGATCA T^GATCA
1346 TGATCA
MaeI 127 CTAG C^TAG
1357 CTAG
MaeII 1427 ACGT A^CGT
1580 ACGT
NcoI 795 CCATGG C^CATGG
1407 CCATGG
PflMI 975 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1181 CCACAGTGTGG
TfiI 473 GATTC G^A[AT]TC
641 GATTC
Van91I 975 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1181 CCACAGTGTGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaII BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrDI BsrFI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI XcmI XhoII