Restriction map for
pF1KB4286
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII Hin1I HindIII
ItaI KasI KpnI KspI MaeI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TseI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 450 AAATTT [GA]^AATT[CT]
ApoI 450 AAATTT [GA]^AATT[CT]
BalI 604 TGGCCA TGG^CCA
BamHI 686 GGATCC G^GATCC
BcnI 368 CCGGG CC^[GC]GG
BpmI 340 CTCCAG CTGGAG(16/14)
BsmI 261 GAATGC GAATGC(1/-1)
BspHI 176 TCATGA T^CATGA
BstAPI 634 GCAATTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 686 GGATCC [GA]^GATC[CT]
CfoI 210 GCGC GCG^C
DdeI 326 CTCAG C^T[ACGT]AG
DrdI 234 GACCGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 644 CCATGG C^C[GA][CT]GG
EcoT14I 644 CCATGG C^C[AT][AT]GG
HgaI 265 GCGTC GACGC(5/10)
HhaI 210 GCGC GCG^C
HinP1I 210 GCGC G^CGC
HincII 352 GTTAAC GT[CT]^[GA]AC
HindII 352 GTTAAC GT[CT]^[GA]AC
HpaI 352 GTTAAC GTT^AAC
MaeIII 109 GTCAC ^GT[ACGT]AC
MfeI 635 CAATTG C^AATTG
MflI 686 GGATCC [GA]^GATC[CT]
MluNI 604 TGGCCA TGG^CCA
MscI 604 TGGCCA TGG^CCA
MslI 174 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 297 CAGCTG C[AC]G^C[GT]G
MunI 635 CAATTG C^AATTG
NciI 368 CCGGG CC^[GC]GG
NcoI 644 CCATGG C^CATGG
NspI 218 ACATGC [GA]CATG^[CT]
PleI 241 GAGTC GAGTC(4/5)
PvuII 297 CAGCTG CAG^CTG
RcaI 176 TCATGA T^CATGA
SfcI 570 CTACAG C^T[GA][CT]AG
SspI 58 AATATT AAT^ATT
StyI 644 CCATGG C^C[AT][AT]GG
Tsp45I 109 GTCAC ^GT[GC]AC
XhoII 686 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 168 GTGCTC G[AT]GC[AT]^C
423 GTGCTC
BmyI 168 GTGCTC G[AGT]GC[ACT]^C
423 GTGCTC
BseRI 379 CTCCTC GAGGAG(10/8)
382 CTCCTC
BsiHKAI 168 GTGCTC G[AT]GC[AT]^C
423 GTGCTC
BsmAI 400 GAGAC GTCTC(1/5)
243 GTCTC
Bsp1286I 168 GTGCTC G[AGT]GC[ACT]^C
423 GTGCTC
BsrI 153 ACTGG ACTGG(1/-1)
14 CCAGT
BstXI 307 CCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
464 CCATGTCCCTGG
DpnII 687 GATC ^GATC
696 GATC
EaeI 18 TGGCCG [CT]^GGCC[GA]
604 TGGCCA
HaeIII 19 GGCC GG^CC
605 GGCC
HapII 21 CCGG C^CGG
368 CCGG
HpaII 21 CCGG C^CGG
368 CCGG
HphI 474 GGTGA GGTGA(8/7)
648 GGTGA
MaeII 350 ACGT A^CGT
581 ACGT
MboI 687 GATC ^GATC
696 GATC
MseI 284 TTAA T^TAA
353 TTAA
MspI 21 CCGG C^CGG
368 CCGG
NdeII 687 GATC ^GATC
696 GATC
NlaIV 77 GGGACC GG[ACGT]^[ACGT]CC
686 GGATCC
Sau3AI 687 GATC ^GATC
696 GATC
TfiI 197 GAATC G^A[AT]TC
460 GAATC
Tru9I 284 TTAA T^TAA
353 TTAA
TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^
116 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI AlwI AvaII BsaJI
BsiYI BslI BsmFI BssKI BstNI
Cfr13I EarI EcoRII HinfI Ksp632I
MboII MnlI MvaI MwoI NlaIII
RsaI Sau96I ScrFI Tsp509I