Restriction map for
pF1KB4240
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 697 GGTACC G^GTACC
AccI 723 GTCTAC GT^[AC][GT]AC
AcsI 612 GAATTC [GA]^AATT[CT]
AflIII 1095 ACGTGT A^C[GA][CT]GT
Aor51HI 542 AGCGCT AGC^GCT
ApoI 612 GAATTC [GA]^AATT[CT]
Asp700I 612 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 697 GGTACC G^GTACC
AspI 505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 531 GAGCCC G[GA]GC[CT]^C
BglI 1366 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1399 GCTAAGC GC^T[ACGT]AGC
BpmI 1181 CTGGAG CTGGAG(16/14)
Bpu1102I 1399 GCTAAGC GC^T[ACGT]AGC
BsaAI 872 TACGTA [CT]AC^GT[GA]
BsmFI 1275 GTCCC GGGAC(10/14)
BsrDI 1316 GCAATG GCAATG(2/0)
BstYI 1285 AGATCC [GA]^GATC[CT]
CelII 1399 GCTAAGC GC^T[ACGT]AGC
DraI 490 TTTAAA TTT^AAA
DraII 802 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 657 GACCACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1251 CCATGG C^C[GA][CT]GG
Eco47III 542 AGCGCT AGC^GCT
EcoNI 580 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 802 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 612 GAATTC G^AATTC
EcoT14I 1251 CCATGG C^C[AT][AT]GG
KpnI 697 GGTACC GGTAC^C
MflI 1285 AGATCC [GA]^GATC[CT]
NcoI 1251 CCATGG C^CATGG
NspI 1230 GCATGC [GA]CATG^[CT]
PflFI 505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 802 AGGACCC [GA]G^G[AT]CC[CT]
PstI 359 CTGCAG CTGCA^G
SapI 545 GCTCTTC GCTCTTC(1/4)
SexAI 661 ACCAGGT A^CC[AT]GGT
SnaBI 872 TACGTA TAC^GTA
SphI 1230 GCATGC GCATG^C
StyI 1251 CCATGG C^C[AT][AT]GG
TaqI 1349 TCGA T^CGA
Tth111I 505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1349 TCGA T^CGA
XhoII 1285 AGATCC [GA]^GATC[CT]
XmnI 612 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BfaI 791 CTAG C^TAG
1120 CTAG
BmyI 192 GGGCAC G[AGT]GC[ACT]^C
531 GAGCCC
BsgI 68 CTGCAC GTGCAG(16/14)
1391 CTGCAC
Bsp1286I 192 GGGCAC G[AGT]GC[ACT]^C
531 GAGCCC
BsrI 38 ACTGG ACTGG(1/-1)
482 CCAGT
Cac8I 1230 GCATGC GC[ACGT]^[ACGT]GC
1362 GCTTGC
CfoI 131 GCGC GCG^C
543 GCGC
HaeII 130 AGCGCC [GA]GCGC^[CT]
542 AGCGCT
HaeIII 1082 GGCC GG^CC
1137 GGCC
HhaI 131 GCGC GCG^C
543 GCGC
HinP1I 131 GCGC G^CGC
543 GCGC
MaeI 791 CTAG C^TAG
1120 CTAG
MaeII 873 ACGT A^CGT
1095 ACGT
MslI 837 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1046 CATCCAAATG
MspA1I 242 CAGCTG C[AC]G^C[GT]G
377 CAGCTG
PleI 897 GACTC GAGTC(4/5)
1310 GACTC
PvuII 242 CAGCTG CAG^CTG
377 CAGCTG
SfcI 305 CTGTAG C^T[GA][CT]AG
359 CTGCAG
XcmI 1077 CCACAGGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1140 CCAAGCTAAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvCI BbvI BsaJI BseRI
BsiYI BslI BsmAI BspMI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI