Restriction map for
pF1KB4190
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 123 CGCG CG^CG
AccIII 1044 TCCGGA T^CCGGA
AcyI 683 GGCGCC G[GA]^CG[CT]C
AocI 330 CCTCAGG CC^T[ACGT]AGG
Aor51HI 274 AGCGCT AGC^GCT
ApaI 8 GGGCCC GGGCC^C
AvaI 745 CTCGGG C^[CT]CG[GA]G
BamHI 29 GGATCC G^GATCC
BbeI 683 GGCGCC GGCGC^C
BbvCI 270 CCTCAGC CCTCAGC(-5/-2)
BcgI 593 GCACCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 380 TGATCA T^GATCA
BfaI 614 CTAG C^TAG
BglI 821 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 844 TACGTG [CT]AC^GT[GA]
BsaHI 683 GGCGCC G[GA]^CG[CT]C
BsaWI 1044 TCCGGA [AT]^CCGG[AT]
BseAI 1044 TCCGGA T^CCGGA
BsgI 34 CTGCAC GTGCAG(16/14)
BsoBI 745 CTCGGG C^[CT]CG[GA]G
BspEI 1044 TCCGGA T^CCGGA
BsrBI 438 CCGCTC CCGCTC(-3/-3)
BsrDI 113 CATTGC GCAATG(2/0)
BstUI 123 CGCG CG^CG
BstXI 155 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 29 GGATCC [GA]^GATC[CT]
Bsu36I 330 CCTCAGG CC^T[ACGT]AGG
DraIII 886 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 274 AGCGCT AGC^GCT
Eco81I 330 CCTCAGG CC^T[ACGT]AGG
EcoNI 1013 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 380 TGATCA T^GATCA
Hin1I 683 GGCGCC G[GA]^CG[CT]C
HincII 706 GTCAAC GT[CT]^[GA]AC
HindII 706 GTCAAC GT[CT]^[GA]AC
HinfI 1041 GACTC G^A[ACGT]TC
KasI 683 GGCGCC G^GCGCC
MaeI 614 CTAG C^TAG
MflI 29 GGATCC [GA]^GATC[CT]
MroI 1044 TCCGGA T^CCGGA
MseI 173 TTAA T^TAA
MvnI 123 CGCG CG^CG
NarI 683 GGCGCC GG^CGCC
NheI 613 GCTAGC G^CTAGC
PflMI 237 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1041 GACTC GAGTC(4/5)
PvuII 778 CAGCTG CAG^CTG
SapI 1074 GAAGAGC GCTCTTC(1/4)
SfoI 683 GGCGCC GGC^GCC
Tru9I 173 TTAA T^TAA
Van91I 237 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 314 CCAGCTTGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 29 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 180 GTAC GT^AC
408 GTAC
AlwI 30 GATCC GGATC(4/5)
29 GGATC
AspHI 655 GTGCTC G[AT]GC[AT]^C
934 GTGCTC
BanII 8 GGGCCC G[GA]GC[CT]^C
1051 GAGCCC
BcnI 50 CCGGG CC^[GC]GG
660 CCCGG
BsaI 949 GAGACC GGTCTC(1/5)
132 GGTCTC
BsiHKAI 655 GTGCTC G[AT]GC[AT]^C
934 GTGCTC
BstEII 147 GGTCACC G^GT[ACGT]ACC
246 GGTGACC
BstF5I 717 CATCC GGATG(2/0)
803 GGATG
BstPI 147 GGTCACC G^GT[ACGT]ACC
246 GGTGACC
EarI 721 CTCTTC CTCTTC(1/4)
1074 GAAGAG
EcoO65I 147 GGTCACC G^GT[ACGT]ACC
246 GGTGACC
FauI 341 CCCGC CCCGC(4/6)
108 GCGGG
FokI 717 CATCC GGATG(9/13)
803 GGATG
HgaI 810 GACGC GACGC(5/10)
347 GCGTC
Ksp632I 721 CTCTTC CTCTTC(1/4)
1074 GAAGAG
MspA1I 778 CAGCTG C[AC]G^C[GT]G
877 CCGCTG
NciI 50 CCGGG CC^[GC]GG
660 CCCGG
NcoI 161 CCATGG C^CATGG
1082 CCATGG
PstI 68 CTGCAG CTGCA^G
856 CTGCAG
RsaI 180 GTAC GT^AC
408 GTAC
SfaNI 677 GCATC GCATC(5/9)
716 GCATC
SfcI 68 CTGCAG C^T[GA][CT]AG
856 CTGCAG
SmlI 403 CTCAAG C^T[CT][GA]AG
484 CTCAAG
StuI 267 AGGCCT AGG^CCT
908 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbsI BbvI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TseI Tsp45I TspRI