Restriction map for
pF1KB4178
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HaeII
Hin1I HpaI HphI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcsI 920 AAATTT [GA]^AATT[CT]
AfaI 108 GTAC GT^AC
AflII 706 CTTAAG C^TTAAG
AflIII 104 ACGTGT A^C[GA][CT]GT
AlwI 363 GGATC GGATC(4/5)
ApaI 181 GGGCCC GGGCC^C
ApoI 920 AAATTT [GA]^AATT[CT]
BanI 166 GGCACC G^G[CT][GA]CC
BfrI 706 CTTAAG C^TTAAG
BsmFI 894 GGGAC GGGAC(10/14)
BspMI 122 ACCTGC ACCTGC(4/8)
BsrBI 154 CCGCTC CCGCTC(-3/-3)
BstF5I 815 GGATG GGATG(2/0)
DsaI 248 CCGCGG C^C[GA][CT]GG
EcoT14I 860 CCTTGG C^C[AT][AT]GG
FokI 815 GGATG GGATG(9/13)
FseI 787 GGCCGGCC GGCCGG^CC
HincII 1015 GTTGAC GT[CT]^[GA]AC
HindII 1015 GTTGAC GT[CT]^[GA]AC
KspI 248 CCGCGG CCGC^GG
MseI 707 TTAA T^TAA
MspA1I 248 CCGCGG C[AC]G^C[GT]G
NlaIII 1012 CATG CATG^
RsaI 108 GTAC GT^AC
SacII 248 CCGCGG CCGC^GG
SapI 205 GAAGAGC GCTCTTC(1/4)
SfaNI 730 GCATC GCATC(5/9)
StyI 860 CCTTGG C^C[AT][AT]GG
Tru9I 707 TTAA T^TAA
XbaI 327 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 211 CGCG CG^CG
249 CGCG
BbsI 16 GAAGAC GAAGAC(2/6)
562 GAAGAC
BglII 354 AGATCT A^GATCT
469 AGATCT
BpmI 195 CTCCAG CTGGAG(16/14)
623 CTCCAG
BpuAI 16 GAAGAC GAAGAC(2/6)
562 GAAGAC
BseRI 479 CTCCTC GAGGAG(10/8)
820 GAGGAG
BsiEI 83 CGGCCG CG[GA][CT]^CG
148 CGGCCG
BsrI 197 CCAGT ACTGG(1/-1)
625 CCAGT
BstUI 211 CGCG CG^CG
249 CGCG
BstYI 354 AGATCT [GA]^GATC[CT]
469 AGATCT
CfoI 96 GCGC GCG^C
210 GCGC
DraII 180 GGGGCCC [GA]G^G[ACGT]CC[CT]
181 GGGCCCC
DrdI 19 GACCCCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
103 GACGTGTACGTC
EagI 83 CGGCCG C^GGCCG
148 CGGCCG
EclXI 83 CGGCCG C^GGCCG
148 CGGCCG
Eco52I 83 CGGCCG C^GGCCG
148 CGGCCG
EcoO109I 180 GGGGCCC [GA]G^G[ACGT]CC[CT]
181 GGGCCCC
FauI 160 CCCGC CCCGC(4/6)
250 GCGGG
HgaI 75 GACGC GACGC(5/10)
896 GACGC
HhaI 96 GCGC GCG^C
210 GCGC
HinP1I 96 GCGC G^CGC
210 GCGC
HindIII 433 AAGCTT A^AGCTT
583 AAGCTT
MaeII 104 ACGT A^CGT
110 ACGT
MflI 354 AGATCT [GA]^GATC[CT]
469 AGATCT
MvnI 211 CGCG CG^CG
249 CGCG
PstI 124 CTGCAG CTGCA^G
782 CTGCAG
SacI 340 GAGCTC GAGCT^C
577 GAGCTC
SfcI 124 CTGCAG C^T[GA][CT]AG
782 CTGCAG
XhoII 354 AGATCT [GA]^GATC[CT]
469 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BssKI BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIV
PleI Sau3AI Sau96I ScrFI SmlI
TfiI TseI Tsp45I Tsp509I TspRI