##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FokI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwI          235  GATCC                GGATC(4/5)
AlwNI         233  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         316  GGACC                G^G[AT]CC
BbvI           10  GCTGC                GCAGC(8/12)
BciVI         369  GTATCC               GTATCC(6/5)
BclI          264  TGATCA               T^GATCA
BsaAI         102  TACGTA               [CT]AC^GT[GA]
BsaJI         226  CCTCGG               C^C[ACGT][ACGT]GG
BseRI         110  CTCCTC               GAGGAG(10/8)
BsiYI         186  CCCTTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          186  CCCTTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI           61  CCAGT                ACTGG(1/-1)
BstYI         234  AGATCC               [GA]^GATC[CT]
Cac8I         144  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I        316  GGACC                G^G[ACGT]CC
DraII         315  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          229  CGGCCA               [CT]^GGCC[GA]
EcoO109I      315  AGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          264  TGATCA               T^GATCA
Fnu4HI         10  GCTGC                GC^[ACGT]GC
HaeIII        230  GGCC                 GG^CC
HphI           24  GGTGA                GGTGA(8/7)
ItaI           10  GCTGC                GC^[ACGT]GC
MflI          234  AGATCC               [GA]^GATC[CT]
MslI          244  CATAATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI            4  GCTGTAGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          203  CATATG               CA^TATG
PpuMI         315  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           11  CTGCAG               CTGCA^G
Sau96I        316  GGACC                G^G[ACGT]CC
SmlI          307  CTTGAG               C^T[CT][GA]AG
SnaBI         102  TACGTA               TAC^GTA
TseI           10  GCTGC                G^C[AT]GC
Tsp509I       130  AATT                 ^AATT
XhoII         234  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

DdeI          334  CTTAG                C^T[ACGT]AG
              356  CTAAG               
DpnII         235  GATC                 ^GATC
              265  GATC                
HinfI         402  GAATC                G^A[ACGT]TC
              408  GAATC               
MaeII         103  ACGT                 A^CGT
              137  ACGT                
MboI          235  GATC                 ^GATC
              265  GATC                
MboII         253  GAAGA                GAAGA(8/7)
              289  GAAGA               
NdeII         235  GATC                 ^GATC
              265  GATC                
NlaIII        262  CATG                 CATG^
              376  CATG                
Sau3AI        235  GATC                 ^GATC
              265  GATC                
SfcI            5  CTGTAG               C^T[GA][CT]AG
               11  CTGCAG              
TfiI          402  GAATC                G^A[AT]TC
              408  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        MnlI