Restriction map for
pF1KB4120
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BclI
BfrI BglI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI HphI KasI KpnI
KspI MaeII MluI MroI MspA1I
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 987 AAATTC [GA]^AATT[CT]
AfaI 214 GTAC GT^AC
ApaI 1154 GGGCCC GGGCC^C
ApoI 987 AAATTC [GA]^AATT[CT]
BalI 203 TGGCCA TGG^CCA
BamHI 1143 GGATCC G^GATCC
BbsI 261 GTCTTC GAAGAC(2/6)
BcgI 864 GCAGATATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 910 GGATAC GTATCC(6/5)
BpmI 217 CTCCAG CTGGAG(16/14)
BpuAI 261 GTCTTC GAAGAC(2/6)
BsaBI 1138 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 576 GGGAC GGGAC(10/14)
BsmI 773 GCATTC GAATGC(1/-1)
BspDI 421 ATCGAT AT^CGAT
BspMI 378 ACCTGC ACCTGC(4/8)
BsrDI 780 CATTGC GCAATG(2/0)
Cac8I 492 GCCTGC GC[ACGT]^[ACGT]GC
ClaI 421 ATCGAT AT^CGAT
DraI 693 TTTAAA TTT^AAA
DraII 1154 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 713 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 450 CCATGG C^C[GA][CT]GG
EaeI 203 TGGCCA [CT]^GGCC[GA]
EarI 317 GAAGAG CTCTTC(1/4)
EcoO109I 1154 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 197 ATGCAT ATGCA^T
HindIII 134 AAGCTT A^AGCTT
Ksp632I 317 GAAGAG CTCTTC(1/4)
MamI 1138 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 992 CAATTG C^AATTG
MluNI 203 TGGCCA TGG^CCA
MscI 203 TGGCCA TGG^CCA
MunI 992 CAATTG C^AATTG
MwoI 175 GCTAGTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 450 CCATGG C^CATGG
NdeI 1130 CATATG CA^TATG
NsiI 197 ATGCAT ATGCA^T
PflMI 219 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 214 GTAC GT^AC
SfaNI 688 GCATC GCATC(5/9)
SmaI 822 CCCGGG CCC^GGG
TfiI 1330 GAATC G^A[AT]TC
Van91I 219 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 822 CCCGGG C^CCGGG
XmaI 822 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 541 CCGC CCGC(-3/-1)
1015 CCGC
AvaI 80 CTCGGG C^[CT]CG[GA]G
822 CCCGGG
AvaII 814 GGACC G^G[AT]CC
970 GGTCC
BanII 825 GGGCTC G[GA]GC[CT]^C
1154 GGGCCC
BbvI 9 GCTGC GCAGC(8/12)
1105 GCTGC
BlpI 827 GCTCAGC GC^T[ACGT]AGC
949 GCTCAGC
BmyI 825 GGGCTC G[AGT]GC[ACT]^C
1154 GGGCCC
Bpu1102I 827 GCTCAGC GC^T[ACGT]AGC
949 GCTCAGC
BsaI 145 GAGACC GGTCTC(1/5)
702 GAGACC
BsmAI 145 GAGAC GTCTC(1/5)
702 GAGAC
BsoBI 80 CTCGGG C^[CT]CG[GA]G
822 CCCGGG
Bsp1286I 825 GGGCTC G[AGT]GC[ACT]^C
1154 GGGCCC
BstF5I 438 GGATG GGATG(2/0)
1212 GGATG
BstYI 32 AGATCC [GA]^GATC[CT]
1143 GGATCC
CelII 827 GCTCAGC GC^T[ACGT]AGC
949 GCTCAGC
CfoI 52 GCGC GCG^C
393 GCGC
EcoRV 229 GATATC GAT^ATC
867 GATATC
EcoT14I 450 CCATGG C^C[AT][AT]GG
706 CCAAGG
Fnu4HI 9 GCTGC GC^[ACGT]GC
1105 GCTGC
FokI 438 GGATG GGATG(9/13)
1212 GGATG
HaeII 51 GGCGCT [GA]GCGC^[CT]
392 AGCGCC
HapII 564 CCGG C^CGG
823 CCGG
HhaI 52 GCGC GCG^C
393 GCGC
HinP1I 52 GCGC G^CGC
393 GCGC
HpaII 564 CCGG C^CGG
823 CCGG
ItaI 9 GCTGC GC^[ACGT]GC
1105 GCTGC
MflI 32 AGATCC [GA]^GATC[CT]
1143 GGATCC
MspI 564 CCGG C^CGG
823 CCGG
PstI 10 CTGCAG CTGCA^G
1106 CTGCAG
StyI 450 CCATGG C^C[AT][AT]GG
706 CCAAGG
TseI 9 GCTGC G^C[AT]GC
1105 GCTGC
Tsp45I 931 GTGAC ^GT[GC]AC
1300 GTGAC
XhoII 32 AGATCC [GA]^GATC[CT]
1143 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI AlwNI BcnI BfaI
BsaJI BseRI BsiYI BslI BsrI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII HaeIII HinfI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfcI
TaqI Tru9I Tsp509I TspRI TthHB8I