Restriction map for
pF1KE0968
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BspDI
BspEI BspHI BspMI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstPI
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 180 CGCG CG^CG
AclI 1540 AACGTT AA^CGTT
AcsI 235 AAATTT [GA]^AATT[CT]
AocI 1242 CCTGAGG CC^T[ACGT]AGG
ApoI 235 AAATTT [GA]^AATT[CT]
BamHI 60 GGATCC G^GATCC
BfaI 44 CTAG C^TAG
BsaAI 786 CACGTA [CT]AC^GT[GA]
BseRI 73 GAGGAG GAGGAG(10/8)
BsmI 1383 GCATTC GAATGC(1/-1)
Bsp1407I 890 TGTACA T^GTACA
BspLU11I 857 ACATGT A^CATGT
BsrDI 297 CATTGC GCAATG(2/0)
BsrGI 890 TGTACA T^GTACA
BsrI 1443 ACTGG ACTGG(1/-1)
Bst1107I 781 GTATAC GTA^TAC
BstF5I 792 CATCC GGATG(2/0)
BstUI 180 CGCG CG^CG
BstZ17I 781 GTATAC GTA^TAC
Bsu36I 1242 CCTGAGG CC^T[ACGT]AGG
CfoI 624 GCGC GCG^C
Eco81I 1242 CCTGAGG CC^T[ACGT]AGG
FokI 792 CATCC GGATG(9/13)
HaeII 623 GGCGCT [GA]GCGC^[CT]
HgaI 1450 GACGC GACGC(5/10)
HhaI 624 GCGC GCG^C
HinP1I 624 GCGC G^CGC
HindIII 127 AAGCTT A^AGCTT
MaeI 44 CTAG C^TAG
MvnI 180 CGCG CG^CG
PaeR7I 1308 CTCGAG C^TCGAG
PleI 102 GACTC GAGTC(4/5)
Psp1406I 1540 AACGTT AA^CGTT
SfcI 1343 CTACAG C^T[GA][CT]AG
SmaI 913 CCCGGG CCC^GGG
SphI 1188 GCATGC GCATG^C
SspBI 890 TGTACA T^GTACA
XhoI 1308 CTCGAG C^TCGAG
XmaCI 913 CCCGGG C^CCGGG
XmaI 913 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 781 GTATAC GT^[AC][GT]AC
1447 GTAGAC
AfaI 789 GTAC GT^AC
891 GTAC
AflIII 785 ACACGT A^C[GA][CT]GT
857 ACATGT
AlwNI 430 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1237 CAGACCCTG
AspHI 13 GAGCTC G[AT]GC[AT]^C
550 GAGCTC
AvaI 913 CCCGGG C^[CT]CG[GA]G
1308 CTCGAG
BalI 353 TGGCCA TGG^CCA
848 TGGCCA
BanII 13 GAGCTC G[GA]GC[CT]^C
550 GAGCTC
BclI 291 TGATCA T^GATCA
1392 TGATCA
BmyI 13 GAGCTC G[AGT]GC[ACT]^C
550 GAGCTC
BsiHKAI 13 GAGCTC G[AT]GC[AT]^C
550 GAGCTC
BsoBI 913 CCCGGG C^[CT]CG[GA]G
1308 CTCGAG
Bsp1286I 13 GAGCTC G[AGT]GC[ACT]^C
550 GAGCTC
BstXI 356 CCAAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1112 CCAGCTTCCTGG
BstYI 60 GGATCC [GA]^GATC[CT]
449 AGATCC
EaeI 353 TGGCCA [CT]^GGCC[GA]
848 TGGCCA
EarI 1157 CTCTTC CTCTTC(1/4)
414 GAAGAG
EcoT14I 65 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
FbaI 291 TGATCA T^GATCA
1392 TGATCA
Ksp632I 1157 CTCTTC CTCTTC(1/4)
414 GAAGAG
MaeIII 81 GTCAC ^GT[ACGT]AC
100 GTGAC
MflI 60 GGATCC [GA]^GATC[CT]
449 AGATCC
MluNI 353 TGGCCA TGG^CCA
848 TGGCCA
MscI 353 TGGCCA TGG^CCA
848 TGGCCA
NaeI 3 GCCGGC GCC^GGC
973 GCCGGC
NdeI 152 CATATG CA^TATG
502 CATATG
NgoMIV 3 GCCGGC G^CCGGC
973 GCCGGC
RsaI 789 GTAC GT^AC
891 GTAC
SacI 13 GAGCTC GAGCT^C
550 GAGCTC
StyI 65 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
TaqI 218 TCGA T^CGA
1309 TCGA
Tsp45I 81 GTCAC ^GT[GC]AC
100 GTGAC
Tsp509I 236 AATT ^AATT
1255 AATT
TspRI 682 CACTG CA[GC]TG[ACGT][ACGT]^
1425 CACTG
TthHB8I 218 TCGA T^CGA
1309 TCGA
XhoII 60 GGATCC [GA]^GATC[CT]
449 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmAI BsrFI BssKI BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I TseI