Restriction map for
pF1KE0954
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Van91I XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 75 CGCG CG^CG
AclI 166 AACGTT AA^CGTT
AlwI 793 GGATC GGATC(4/5)
AlwNI 1035 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 732 TGGCCA TGG^CCA
BbvCI 1125 CCTCAGC CCTCAGC(-5/-2)
BglI 1138 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 478 CTGGAG CTGGAG(16/14)
BsaAI 1165 TACGTG [CT]AC^GT[GA]
BsaI 154 GAGACC GGTCTC(1/5)
BsaWI 818 ACCGGA [AT]^CCGG[AT]
BseRI 1054 GAGGAG GAGGAG(10/8)
BsgI 964 GTGCAG GTGCAG(16/14)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmBI 28 GAGACG CGTCTC(1/5)
BsrBI 355 GAGCGG CCGCTC(-3/-3)
BsrDI 323 GCAATG GCAATG(2/0)
BsrFI 1098 GCCGGT [GA]^CCGG[CT]
BstUI 75 CGCG CG^CG
Cfr10I 1098 GCCGGT [GA]^CCGG[CT]
DrdI 1407 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 300 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 74 CCGCGG CCGC^GG
MaeIII 673 GTCAC ^GT[ACGT]AC
MluNI 732 TGGCCA TGG^CCA
MscI 732 TGGCCA TGG^CCA
MslI 1161 CACATACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 75 CGCG CG^CG
NcoI 830 CCATGG C^CATGG
PflFI 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 79 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 166 AACGTT AA^CGTT
PstI 1045 CTGCAG CTGCA^G
PvuII 107 CAGCTG CAG^CTG
SacII 74 CCGCGG CCGC^GG
SapI 465 GCTCTTC GCTCTTC(1/4)
SfcI 1045 CTGCAG C^T[GA][CT]AG
SgrAI 1097 CGCCGGTG C[GA]^CCGG[CT]G
StuI 1145 AGGCCT AGG^CCT
TaqI 1369 TCGA T^CGA
Tsp45I 673 GTCAC ^GT[GC]AC
Tth111I 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1369 TCGA T^CGA
XbaI 366 TCTAGA T^CTAGA
XcmI 695 CCATTGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 702 GTAC GT^AC
777 GTAC
AocI 300 CCTAAGG CC^T[ACGT]AGG
1400 CCTCAGG
ApaI 187 GGGCCC GGGCC^C
767 GGGCCC
AspHI 64 GAGCTC G[AT]GC[AT]^C
463 GAGCTC
AviII 320 TGCGCA TGC^GCA
725 TGCGCA
BfaI 115 CTAG C^TAG
367 CTAG
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
463 GAGCTC
BstXI 414 CCAGACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCAGCGGATTGG
Bsu36I 300 CCTAAGG CC^T[ACGT]AGG
1400 CCTCAGG
EaeI 5 CGGCCG [CT]^GGCC[GA]
732 TGGCCA
EarI 466 CTCTTC CTCTTC(1/4)
136 GAAGAG
Eco81I 300 CCTAAGG CC^T[ACGT]AGG
1400 CCTCAGG
EcoT14I 329 CCAAGG C^C[AT][AT]GG
830 CCATGG
FauI 73 CCCGC CCCGC(4/6)
1079 CCCGC
FspI 320 TGCGCA TGC^GCA
725 TGCGCA
HphI 875 TCACC GGTGA(8/7)
1228 TCACC
Ksp632I 466 CTCTTC CTCTTC(1/4)
136 GAAGAG
MaeI 115 CTAG C^TAG
367 CTAG
NspI 82 GCATGT [GA]CATG^[CT]
782 ACATGC
RsaI 702 GTAC GT^AC
777 GTAC
SacI 64 GAGCTC GAGCT^C
463 GAGCTC
SmaI 887 CCCGGG CCC^GGG
1253 CCCGGG
StyI 329 CCAAGG C^C[AT][AT]GG
830 CCATGG
TfiI 717 GATTC G^A[AT]TC
918 GAATC
Tsp509I 315 AATT ^AATT
994 AATT
XmaCI 887 CCCGGG C^CCGGG
1253 CCCGGG
XmaI 887 CCCGGG C^CCGGG
1253 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AluI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BlpI BmyI Bpu1102I BsaHI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SfoI SmlI TseI
TspRI