Restriction map for
pF1KE0768
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 981 GAATTT [GA]^AATT[CT]
AflIII 1115 ACGTGT A^C[GA][CT]GT
Alw44I 1119 GTGCAC G^TGCAC
AlwI 310 GGATC GGATC(4/5)
AlwNI 316 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 63 AGCGCT AGC^GCT
ApaI 715 GGGCCC GGGCC^C
ApaLI 1119 GTGCAC G^TGCAC
ApoI 981 GAATTT [GA]^AATT[CT]
Asp700I 1191 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1271 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1004 TGGCCA TGG^CCA
BbrPI 1114 CACGTG CAC^GTG
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BciVI 585 GGATAC GTATCC(6/5)
BsaAI 1114 CACGTG [CT]AC^GT[GA]
BsaBI 77 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 867 GAGACC GGTCTC(1/5)
BsgI 293 CTGCAC GTGCAG(16/14)
BsmI 704 GAATGC GAATGC(1/-1)
Bsp1407I 93 TGTACA T^GTACA
BsrBI 142 GAGCGG CCGCTC(-3/-3)
BsrGI 93 TGTACA T^GTACA
BstEII 324 GGTAACC G^GT[ACGT]ACC
BstPI 324 GGTAACC G^GT[ACGT]ACC
BstXI 1050 CCAAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 64 GCGC GCG^C
DpnII 311 GATC ^GATC
DraIII 1156 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 148 CCATGG C^C[GA][CT]GG
Eco47III 63 AGCGCT AGC^GCT
EcoNI 192 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 324 GGTAACC G^GT[ACGT]ACC
EcoT14I 148 CCATGG C^C[AT][AT]GG
HaeII 63 AGCGCT [GA]GCGC^[CT]
HhaI 64 GCGC GCG^C
HinP1I 64 GCGC G^CGC
MaeII 1115 ACGT A^CGT
MamI 77 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 311 GATC ^GATC
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
MseI 781 TTAA T^TAA
NcoI 148 CCATGG C^CATGG
NdeII 311 GATC ^GATC
PflFI 1271 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1114 CACGTG CAC^GTG
PmlI 1114 CACGTG CAC^GTG
PstI 32 CTGCAG CTGCA^G
Sau3AI 311 GATC ^GATC
SmlI 883 CTCAAG C^T[CT][GA]AG
SphI 1023 GCATGC GCATG^C
SspBI 93 TGTACA T^GTACA
StyI 148 CCATGG C^C[AT][AT]GG
Tru9I 781 TTAA T^TAA
Tth111I 1271 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1191 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 94 GTAC GT^AC
160 GTAC
BbsI 894 GAAGAC GAAGAC(2/6)
908 GAAGAC
BcnI 657 CCCGG CC^[GC]GG
1280 CCCGG
BfaI 484 CTAG C^TAG
598 CTAG
BpuAI 894 GAAGAC GAAGAC(2/6)
908 GAAGAC
BstAPI 295 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1034 GCAAATCCTGC
HapII 658 CCGG C^CGG
1281 CCGG
HpaII 658 CCGG C^CGG
1281 CCGG
MaeI 484 CTAG C^TAG
598 CTAG
MslI 762 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
940 CACCTACATG
MspI 658 CCGG C^CGG
1281 CCGG
NciI 657 CCCGG CC^[GC]GG
1280 CCCGG
PpuMI 165 AGGACCT [GA]G^G[AT]CC[CT]
506 GGGTCCC
RsaI 94 GTAC GT^AC
160 GTAC
SfaNI 989 GATGC GCATC(5/9)
843 GCATC
SfcI 32 CTGCAG C^T[GA][CT]AG
873 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
NspI PvuII SapI Sau96I ScrFI
TfiI TseI Tsp45I Tsp509I TspRI