##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeIII      MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwNI         634  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       784  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI         1041  GGTGCC               G^G[CT][GA]CC
BanII         308  GAGCCC               G[GA]GC[CT]^C
BclI          720  TGATCA               T^GATCA
BsaBI         715  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         906  CCAGGG               C^C[ACGT][ACGT]GG
BssKI         906  CCAGG                ^CC[ACGT]GG
BstNI         906  CCAGG                CC^[AT]GG
BstXI        1031  CCATTGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         358  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        241  GGGCC                G^G[ACGT]CC
EarI          802  GAAGAG               CTCTTC(1/4)
EcoNI          36  CCTCCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        906  CCAGG                ^CC[AT]GG
FbaI          720  TGATCA               T^GATCA
HaeIII        242  GGCC                 GG^CC
HincII        758  GTCAAC               GT[CT]^[GA]AC
HindII        758  GTCAAC               GT[CT]^[GA]AC
Ksp632I       802  GAAGAG               CTCTTC(1/4)
MaeII         582  ACGT                 A^CGT
MamI          715  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          323  CAATTG               C^AATTG
MspA1I        637  CAGCTG               C[AC]G^C[GT]G
MunI          323  CAATTG               C^AATTG
MvaI          906  CCAGG                CC^[AT]GG
PleI          846  GAGTC                GAGTC(4/5)
PvuII         637  CAGCTG               CAG^CTG
Sau96I        241  GGGCC                G^G[ACGT]CC
ScrFI         906  CCAGG                CC^[ACGT]GG
SfcI          504  CTACAG               C^T[GA][CT]AG
XmnI          784  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          631  GTAC                 GT^AC
              923  GTAC                
BseRI          21  CTCCTC               GAGGAG(10/8)
              698  GAGGAG              
BstF5I        724  CATCC                GGATG(2/0)
              537  GGATG               
DpnII         385  GATC                 ^GATC
              721  GATC                
FokI          724  CATCC                GGATG(9/13)
              537  GGATG               
MboI          385  GATC                 ^GATC
              721  GATC                
MseI          146  TTAA                 T^TAA
              953  TTAA                
NdeII         385  GATC                 ^GATC
              721  GATC                
RsaI          631  GTAC                 GT^AC
              923  GTAC                
Sau3AI        385  GATC                 ^GATC
              721  GATC                
SfaNI         202  GATGC                GCATC(5/9)
              616  GATGC               
TfiI          136  GAATC                G^A[AT]TC
              664  GAATC               
Tru9I         146  TTAA                 T^TAA
              953  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AcsI        AluI        AocI        ApoI        AspHI       
BbvI        BmyI        BpmI        BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        Bsu36I      DdeI        
Eco81I      Fnu4HI      HinfI       HphI        ItaI        
MboII       MnlI        NlaIV       TseI        Tsp509I     
TspRI