Restriction map for
pF1KE0684
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BcgI BciVI BclI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BseAI BsgI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspHI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SwaI
TspRI Tth111I Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 298 GACGTC GACGT^C
AcyI 298 GACGTC G[GA]^CG[CT]C
AflII 444 CTTAAG C^TTAAG
AocI 694 CCTGAGG CC^T[ACGT]AGG
Asp700I 64 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 982 GAGCTC G[AT]GC[AT]^C
BamHI 237 GGATCC G^GATCC
BbrPI 564 CACGTG CAC^GTG
BfaI 73 CTAG C^TAG
BfrI 444 CTTAAG C^TTAAG
BsaHI 298 GACGTC G[GA]^CG[CT]C
BsaWI 1073 ACCGGA [AT]^CCGG[AT]
BsiHKAI 982 GAGCTC G[AT]GC[AT]^C
Bsp1407I 435 TGTACA T^GTACA
BspLU11I 1157 ACATGT A^CATGT
BsrFI 848 GCCGGT [GA]^CCGG[CT]
BsrGI 435 TGTACA T^GTACA
Bsu36I 694 CCTGAGG CC^T[ACGT]AGG
Cfr10I 848 GCCGGT [GA]^CCGG[CT]
DraIII 619 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1041 CGGCCG [CT]^GGCC[GA]
EagI 1041 CGGCCG C^GGCCG
EarI 55 GAAGAG CTCTTC(1/4)
EclXI 1041 CGGCCG C^GGCCG
Eco52I 1041 CGGCCG C^GGCCG
Eco81I 694 CCTGAGG CC^T[ACGT]AGG
EcoNI 690 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 251 GAATTC G^AATTC
EcoT14I 212 CCATGG C^C[AT][AT]GG
FauI 683 GCGGG CCCGC(4/6)
HaeII 845 AGCGCC [GA]GCGC^[CT]
Hin1I 298 GACGTC G[GA]^CG[CT]C
Ksp632I 55 GAAGAG CTCTTC(1/4)
KspI 666 CCGCGG CCGC^GG
MaeI 73 CTAG C^TAG
MaeIII 629 GTGAC ^GT[ACGT]AC
MspA1I 666 CCGCGG C[AC]G^C[GT]G
NcoI 212 CCATGG C^CATGG
NspI 1157 ACATGT [GA]CATG^[CT]
PleI 189 GACTC GAGTC(4/5)
PmaCI 564 CACGTG CAC^GTG
PmlI 564 CACGTG CAC^GTG
PshAI 76 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 982 GAGCTC GAGCT^C
SacII 666 CCGCGG CCGC^GG
SfcI 280 CTACAG C^T[GA][CT]AG
SgrAI 847 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 444 CTTAAG C^T[CT][GA]AG
SspBI 435 TGTACA T^GTACA
SspI 379 AATATT AAT^ATT
StyI 212 CCATGG C^C[AT][AT]GG
TfiI 917 GAATC G^A[AT]TC
Tsp45I 629 GTGAC ^GT[GC]AC
XbaI 72 TCTAGA T^CTAGA
XmnI 64 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 436 GTAC GT^AC
1071 GTAC
AflIII 563 ACACGT A^C[GA][CT]GT
1157 ACATGT
AvaII 687 GGACC G^G[AT]CC
957 GGACC
BbvCI 936 CCTCAGC CCTCAGC(-5/-2)
705 GCTGAGG
BbvI 471 GCTGC GCAGC(8/12)
702 GCTGC
BsaAI 564 CACGTG [CT]AC^GT[GA]
571 TACGTG
BsiEI 795 CGACCG CG[GA][CT]^CG
1041 CGGCCG
BsmFI 350 GGGAC GGGAC(10/14)
686 GGGAC
BspMI 275 ACCTGC ACCTGC(4/8)
1008 GCAGGT
Cac8I 32 GCGTGC GC[ACGT]^[ACGT]GC
806 GCTGGC
CfoI 484 GCGC GCG^C
846 GCGC
Cfr13I 687 GGACC G^G[ACGT]CC
957 GGACC
DraII 686 GGGACCT [GA]G^G[ACGT]CC[CT]
956 AGGACCC
EcoO109I 686 GGGACCT [GA]G^G[ACGT]CC[CT]
956 AGGACCC
HhaI 484 GCGC GCG^C
846 GCGC
HinP1I 484 GCGC G^CGC
846 GCGC
HinfI 189 GACTC G^A[ACGT]TC
917 GAATC
HphI 141 GGTGA GGTGA(8/7)
361 GGTGA
MslI 588 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
654 CATCGCCATG
PpuMI 686 GGGACCT [GA]G^G[AT]CC[CT]
956 AGGACCC
RsaI 436 GTAC GT^AC
1071 GTAC
Sau96I 687 GGACC G^G[ACGT]CC
957 GGACC
SfaNI 671 GCATC GCATC(5/9)
941 GCATC
SmaI 475 CCCGGG CCC^GGG
541 CCCGGG
StuI 327 AGGCCT AGG^CCT
1019 AGGCCT
TaqI 84 TCGA T^CGA
155 TCGA
TseI 471 GCTGC G^C[AT]GC
702 GCTGC
TthHB8I 84 TCGA T^CGA
155 TCGA
XmaCI 475 CCCGGG C^CCGGG
541 CCCGGG
XmaI 475 CCCGGG C^CCGGG
541 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaI BanII BbsI BcnI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI ScrFI
Tru9I Tsp509I XhoII