Restriction map for
pF1KB7691
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspLU11I BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 99 TCCGGA T^CCGGA
AcsI 355 GAATTT [GA]^AATT[CT]
AlwNI 925 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 355 GAATTT [GA]^AATT[CT]
Asp700I 264 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 537 GAAGAC GAAGAC(2/6)
BfaI 421 CTAG C^TAG
BpmI 736 CTCCAG CTGGAG(16/14)
BpuAI 537 GAAGAC GAAGAC(2/6)
BsaI 376 GGTCTC GGTCTC(1/5)
BsaWI 99 TCCGGA [AT]^CCGG[AT]
BseAI 99 TCCGGA T^CCGGA
Bsp1407I 166 TGTACA T^GTACA
BspEI 99 TCCGGA T^CCGGA
BspMI 592 ACCTGC ACCTGC(4/8)
BsrGI 166 TGTACA T^GTACA
BstAPI 579 GCACACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 15 AGATCC [GA]^GATC[CT]
DraIII 397 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 379 CTCTTC CTCTTC(1/4)
HaeII 424 GGCGCT [GA]GCGC^[CT]
HinfI 69 GATTC G^A[ACGT]TC
Ksp632I 379 CTCTTC CTCTTC(1/4)
KspI 610 CCGCGG CCGC^GG
MaeI 421 CTAG C^TAG
MflI 15 AGATCC [GA]^GATC[CT]
MroI 99 TCCGGA T^CCGGA
NcoI 791 CCATGG C^CATGG
PflMI 396 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 327 CTGCAG CTGCA^G
PvuII 574 CAGCTG CAG^CTG
SacII 610 CCGCGG CCGC^GG
ScaI 876 AGTACT AGT^ACT
SfcI 327 CTGCAG C^T[GA][CT]AG
SphI 114 GCATGC GCATG^C
SspBI 166 TGTACA T^GTACA
TaqI 305 TCGA T^CGA
TfiI 69 GATTC G^A[AT]TC
Tsp45I 902 GTGAC ^GT[GC]AC
TthHB8I 305 TCGA T^CGA
Van91I 396 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 336 CCAGCTCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 15 AGATCC [GA]^GATC[CT]
XmnI 264 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 590 GGACC G^G[AT]CC
952 GGACC
BanII 481 GAGCCC G[GA]GC[CT]^C
957 GGGCTC
BcnI 675 CCGGG CC^[GC]GG
955 CCGGG
BsgI 577 CTGCAC GTGCAG(16/14)
243 GTGCAG
BspHI 626 TCATGA T^CATGA
722 TCATGA
BsrBI 285 CCGCTC CCGCTC(-3/-3)
436 CCGCTC
DsaI 610 CCGCGG C^C[GA][CT]GG
791 CCATGG
EaeI 460 CGGCCA [CT]^GGCC[GA]
613 CGGCCA
MaeII 33 ACGT A^CGT
518 ACGT
MaeIII 857 GTTAC ^GT[ACGT]AC
902 GTGAC
MboII 537 GAAGA GAAGA(8/7)
380 TCTTC
NciI 675 CCGGG CC^[GC]GG
955 CCGGG
NlaIV 480 GGAGCC GG[ACGT]^[ACGT]CC
512 GGCCCC
RcaI 626 TCATGA T^CATGA
722 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp509I TspRI