Restriction map for
pF1KB6486
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BcnI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NarI NciI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tsp45I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 712 CTTAAG C^TTAAG
Asp700I 879 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 973 GAGCTC G[AT]GC[AT]^C
AspI 247 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 80 GGCACC G^G[CT][GA]CC
BanII 973 GAGCTC G[GA]GC[CT]^C
BclI 830 TGATCA T^GATCA
BfaI 904 CTAG C^TAG
BfrI 712 CTTAAG C^TTAAG
BsaI 136 GAGACC GGTCTC(1/5)
BsiHKAI 973 GAGCTC G[AT]GC[AT]^C
BsmBI 21 CGTCTC CGTCTC(1/5)
BsmFI 445 GGGAC GGGAC(10/14)
Bsp1407I 816 TGTACA T^GTACA
BspHI 999 TCATGA T^CATGA
BsrFI 77 GCCGGC [GA]^CCGG[CT]
BsrGI 816 TGTACA T^GTACA
BstAPI 1011 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 77 GCCGGC [GA]^CCGG[CT]
DraII 569 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 658 CCGTGG C^C[GA][CT]GG
EcoNI 492 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 569 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 879 GAATTC G^AATTC
FauI 48 GCGGG CCCGC(4/6)
FbaI 830 TGATCA T^GATCA
HapII 78 CCGG C^CGG
HpaII 78 CCGG C^CGG
MaeI 904 CTAG C^TAG
MaeII 8 ACGT A^CGT
MslI 820 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 78 CCGG C^CGG
NaeI 77 GCCGGC GCC^GGC
NdeI 718 CATATG CA^TATG
NgoMIV 77 GCCGGC G^CCGGC
PflFI 247 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 642 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 569 AGGACCC [GA]G^G[AT]CC[CT]
PstI 283 CTGCAG CTGCA^G
RcaI 999 TCATGA T^CATGA
SacI 973 GAGCTC GAGCT^C
SmlI 712 CTTAAG C^T[CT][GA]AG
SspBI 816 TGTACA T^GTACA
Tth111I 247 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 642 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 879 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 774 CCGC CCGC(-3/-1)
48 GCGG
AcsI 12 GAATTT [GA]^AATT[CT]
879 GAATTC
AlwNI 1009 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1012 CAGCTGCTG
ApoI 12 GAATTT [GA]^AATT[CT]
879 GAATTC
BbvCI 1030 CCTCAGC CCTCAGC(-5/-2)
436 GCTGAGG
BmyI 487 GTGCCC G[AGT]GC[ACT]^C
973 GAGCTC
BpmI 33 CTGGAG CTGGAG(16/14)
540 CTGGAG
Bsp1286I 487 GTGCCC G[AGT]GC[ACT]^C
973 GAGCTC
BstF5I 420 GGATG GGATG(2/0)
965 GGATG
Cac8I 77 GCCGGC GC[ACGT]^[ACGT]GC
1007 GCCAGC
DraIII 304 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
724 CACACTGTG
EarI 346 GAAGAG CTCTTC(1/4)
381 GAAGAG
FokI 420 GGATG GGATG(9/13)
965 GGATG
Ksp632I 346 GAAGAG CTCTTC(1/4)
381 GAAGAG
MseI 713 TTAA T^TAA
860 TTAA
MspA1I 1012 CAGCTG C[AC]G^C[GT]G
1033 CAGCTG
PvuII 1012 CAGCTG CAG^CTG
1033 CAGCTG
SfcI 283 CTGCAG C^T[GA][CT]AG
410 CTATAG
TaqI 165 TCGA T^CGA
923 TCGA
Tru9I 713 TTAA T^TAA
860 TTAA
TthHB8I 165 TCGA T^CGA
923 TCGA
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AvaII BalI BbvI
BsaJI BsiYI BslI BsmAI BspMI
BsrI BssKI BstNI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI