Restriction map for
pF1KB6796
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NlaIV
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TfiI TspRI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 192 GTCTAC GT^[AC][GT]AC
AcsI 104 GAATTC [GA]^AATT[CT]
AfaI 161 GTAC GT^AC
AlwI 87 GATCC GGATC(4/5)
AlwNI 121 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 104 GAATTC [GA]^AATT[CT]
Asp700I 100 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 43 CTCGAG C^[CT]CG[GA]G
BbvI 144 GCAGC GCAGC(8/12)
BciVI 60 GGATAC GTATCC(6/5)
BclI 114 TGATCA T^GATCA
BmyI 360 GTGCCC G[AGT]GC[ACT]^C
BsaJI 56 CCTCGG C^C[ACGT][ACGT]GG
BsoBI 43 CTCGAG C^[CT]CG[GA]G
Bsp1286I 360 GTGCCC G[AGT]GC[ACT]^C
BssKI 90 CCAGG ^CC[ACGT]GG
BstF5I 324 GGATG GGATG(2/0)
BstNI 90 CCAGG CC^[AT]GG
BstYI 86 AGATCC [GA]^GATC[CT]
DdeI 230 CTAAG C^T[ACGT]AG
EarI 197 CTCTTC CTCTTC(1/4)
EcoRI 104 GAATTC G^AATTC
EcoRII 90 CCAGG ^CC[AT]GG
FbaI 114 TGATCA T^GATCA
Fnu4HI 144 GCAGC GC^[ACGT]GC
FokI 324 GGATG GGATG(9/13)
HaeIII 81 GGCC GG^CC
HinfI 190 GAGTC G^A[ACGT]TC
HphI 38 TCACC GGTGA(8/7)
ItaI 144 GCAGC GC^[ACGT]GC
Ksp632I 197 CTCTTC CTCTTC(1/4)
MaeII 159 ACGT A^CGT
MaeIII 401 GTCAC ^GT[ACGT]AC
MflI 86 AGATCC [GA]^GATC[CT]
MseI 293 TTAA T^TAA
MslI 415 CATTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 145 CAGCTG C[AC]G^C[GT]G
MvaI 90 CCAGG CC^[AT]GG
PaeR7I 43 CTCGAG C^TCGAG
PleI 190 GAGTC GAGTC(4/5)
PvuII 145 CAGCTG CAG^CTG
RsaI 161 GTAC GT^AC
ScrFI 90 CCAGG CC^[ACGT]GG
SmlI 43 CTCGAG C^T[CT][GA]AG
Tru9I 293 TTAA T^TAA
TseI 144 GCAGC G^C[AT]GC
Tsp45I 401 GTCAC ^GT[GC]AC
XhoI 43 CTCGAG C^TCGAG
XhoII 86 AGATCC [GA]^GATC[CT]
XmnI 100 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
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AluI 146 AGCT AG^CT
296 AGCT
BbsI 30 GAAGAC GAAGAC(2/6)
460 GAAGAC
BpuAI 30 GAAGAC GAAGAC(2/6)
460 GAAGAC
BsiYI 20 CCCTTACGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
90 CCAGGATAAGG
BslI 20 CCCTTACGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
90 CCAGGATAAGG
BsmAI 123 GAGAC GTCTC(1/5)
213 GAGAC
SspI 178 AATATT AAT^ATT
311 AATATT
TaqI 44 TCGA T^CGA
354 TCGA
Tsp509I 105 AATT ^AATT
339 AATT
TthHB8I 44 TCGA T^CGA
354 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
Cac8I DpnII MboI MboII MnlI
NdeII Sau3AI