Restriction map for
pF1KE3190
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlpI
BmyI BpmI Bpu1102I BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NarI NcoI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1253 CGCG CG^CG
AflIII 1223 ACATGT A^C[GA][CT]GT
AlwNI 1280 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 193 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 30 TGCGCA TGC^GCA
AvrII 177 CCTAGG C^CTAGG
BcnI 277 CCCGG CC^[GC]GG
BfaI 178 CTAG C^TAG
BglI 720 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1175 AGATCT A^GATCT
BlnI 177 CCTAGG C^CTAGG
BsaAI 782 TACGTG [CT]AC^GT[GA]
BsaI 751 GAGACC GGTCTC(1/5)
BsiYI 1022 CCTTATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1022 CCTTATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 381 TGTACA T^GTACA
BspLU11I 1223 ACATGT A^CATGT
BspMI 50 ACCTGC ACCTGC(4/8)
BsrBI 55 CCGCTC CCGCTC(-3/-3)
BsrDI 377 GCAATG GCAATG(2/0)
BsrGI 381 TGTACA T^GTACA
BstUI 1253 CGCG CG^CG
BstYI 1175 AGATCT [GA]^GATC[CT]
CfoI 31 GCGC GCG^C
Cfr13I 1030 GGGCC G^G[ACGT]CC
EcoRI 884 GAATTC G^AATTC
EcoT14I 177 CCTAGG C^C[AT][AT]GG
FseI 1114 GGCCGGCC GGCCGG^CC
FspI 30 TGCGCA TGC^GCA
HgaI 25 GACGC GACGC(5/10)
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
MaeI 178 CTAG C^TAG
MaeII 783 ACGT A^CGT
MaeIII 1227 GTCAC ^GT[ACGT]AC
MfeI 476 CAATTG C^AATTG
MflI 1175 AGATCT [GA]^GATC[CT]
MunI 476 CAATTG C^AATTG
MvnI 1253 CGCG CG^CG
NciI 277 CCCGG CC^[GC]GG
NspI 1223 ACATGT [GA]CATG^[CT]
Sau96I 1030 GGGCC G^G[ACGT]CC
SfaNI 745 GATGC GCATC(5/9)
SspBI 381 TGTACA T^GTACA
SspI 1008 AATATT AAT^ATT
StyI 177 CCTAGG C^C[AT][AT]GG
TaqI 432 TCGA T^CGA
Tsp45I 1227 GTCAC ^GT[GC]AC
TthHB8I 432 TCGA T^CGA
XhoII 1175 AGATCT [GA]^GATC[CT]
XmnI 193 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 55 CCGC CCGC(-3/-1)
330 GCGG
AfaI 382 GTAC GT^AC
953 GTAC
AlwI 267 GATCC GGATC(4/5)
1275 GATCC
BsaBI 261 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
793 GATGAAAATC
BsaJI 177 CCTAGG C^C[ACGT][ACGT]GG
1144 CCTGGG
BsrFI 4 GCCGGC [GA]^CCGG[CT]
1115 GCCGGC
BstF5I 792 GGATG GGATG(2/0)
1195 GGATG
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
1115 GCCGGC
DdeI 508 CTAAG C^T[ACGT]AG
749 CTGAG
EaeI 2 TGGCCG [CT]^GGCC[GA]
1208 CGGCCA
FokI 792 GGATG GGATG(9/13)
1195 GGATG
MamI 261 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
793 GATGAAAATC
MslI 659 CATATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1057 CATGGATATG
MspA1I 228 CAGCTG C[AC]G^C[GT]G
1134 CAGCTG
NaeI 4 GCCGGC GCC^GGC
1115 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
1115 GCCGGC
PshAI 148 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
226 GACAGCTGTC
PstI 65 CTGCAG CTGCA^G
584 CTGCAG
PvuII 228 CAGCTG CAG^CTG
1134 CAGCTG
RsaI 382 GTAC GT^AC
953 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BpuAI BsmAI BsrI BssKI BstNI
Cac8I DpnII EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfcI SmlI
TfiI Tru9I TseI Tsp509I TspRI