Restriction map for
pF1KB6542
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 436 GTAGAC GT^[AC][GT]AC
AvaI 260 CTCGGG C^[CT]CG[GA]G
AvaII 966 GGACC G^G[AT]CC
BbvCI 403 GCTGAGG CCTCAGC(-5/-2)
BcgI 372 GCACTTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 207 GGATAC GTATCC(6/5)
BcnI 537 CCGGG CC^[GC]GG
BsaBI 1033 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 507 GAGACC GGTCTC(1/5)
BsgI 43 CTGCAC GTGCAG(16/14)
BsiYI 815 CCCTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 815 CCCTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 389 GAATGC GAATGC(1/-1)
BsoBI 260 CTCGGG C^[CT]CG[GA]G
BsrFI 106 ACCGGC [GA]^CCGG[CT]
BssSI 121 CACGAG CACGAG(-5/-1)
BstXI 1046 CCATGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 125 AGATCC [GA]^GATC[CT]
Cfr10I 106 ACCGGC [GA]^CCGG[CT]
Cfr13I 966 GGACC G^G[ACGT]CC
EaeI 141 CGGCCA [CT]^GGCC[GA]
EcoRV 781 GATATC GAT^ATC
HaeII 873 GGCGCT [GA]GCGC^[CT]
MamI 1033 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 1014 TCTTC GAAGA(8/7)
MflI 125 AGATCC [GA]^GATC[CT]
MslI 247 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 67 CAGCTG C[AC]G^C[GT]G
NciI 537 CCGGG CC^[GC]GG
NdeI 746 CATATG CA^TATG
NlaIII 1047 CATG CATG^
PacI 886 TTAATTAA TTAAT^TAA
PleI 835 GAGTC GAGTC(4/5)
PshAI 439 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 130 CTGCAG CTGCA^G
PvuII 67 CAGCTG CAG^CTG
Sau96I 966 GGACC G^G[ACGT]CC
SgrAI 105 CACCGGCG C[GA]^CCGG[CT]G
SmlI 115 CTCAAG C^T[CT][GA]AG
SspI 477 AATATT AAT^ATT
XhoII 125 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 25 CGCG CG^CG
79 CGCG
AciI 80 GCGG CCGC(-3/-1)
110 GCGG
AfaI 717 GTAC GT^AC
923 GTAC
AlwI 126 GATCC GGATC(4/5)
3 GGATC
BseRI 88 GAGGAG GAGGAG(10/8)
406 GAGGAG
BsmAI 507 GAGAC GTCTC(1/5)
723 GAGAC
BspMI 510 ACCTGC ACCTGC(4/8)
862 GCAGGT
BsrI 925 ACTGG ACTGG(1/-1)
771 CCAGT
BstUI 25 CGCG CG^CG
79 CGCG
DpnII 4 GATC ^GATC
126 GATC
DsaI 138 CCACGG C^C[GA][CT]GG
830 CCGTGG
EcoT14I 566 CCTTGG C^C[AT][AT]GG
969 CCTTGG
HapII 107 CCGG C^CGG
537 CCGG
HpaII 107 CCGG C^CGG
537 CCGG
MaeIII 13 GTGAC ^GT[ACGT]AC
788 GTGAC
MboI 4 GATC ^GATC
126 GATC
MspI 107 CCGG C^CGG
537 CCGG
MvnI 25 CGCG CG^CG
79 CGCG
NdeII 4 GATC ^GATC
126 GATC
RsaI 717 GTAC GT^AC
923 GTAC
SacI 91 GAGCTC GAGCT^C
409 GAGCTC
Sau3AI 4 GATC ^GATC
126 GATC
SfcI 130 CTGCAG C^T[GA][CT]AG
434 CTGTAG
StyI 566 CCTTGG C^C[AT][AT]GG
969 CCTTGG
TaqI 98 TCGA T^CGA
381 TCGA
TfiI 292 GATTC G^A[AT]TC
421 GAATC
Tsp45I 13 GTGAC ^GT[GC]AC
788 GTGAC
TthHB8I 98 TCGA T^CGA
381 TCGA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AluI ApoI AspHI BanII
BbvI BmyI BpmI BsaJI BsiHKAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI DdeI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MnlI MseI MvaI
MwoI NlaIV ScrFI SfaNI Tru9I
TseI Tsp509I TspRI