Restriction map for
pF1KE0894
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MluI MroI MseI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 366 CCGC CCGC(-3/-1)
AfaI 105 GTAC GT^AC
AlwI 923 GATCC GGATC(4/5)
AlwNI 895 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 143 TGGCCA TGG^CCA
BbrPI 814 CACGTG CAC^GTG
BbsI 270 GAAGAC GAAGAC(2/6)
BbvCI 543 CCTCAGC CCTCAGC(-5/-2)
BbvI 338 GCTGC GCAGC(8/12)
BcnI 59 CCCGG CC^[GC]GG
BpuAI 270 GAAGAC GAAGAC(2/6)
BsaAI 814 CACGTG [CT]AC^GT[GA]
BseRI 54 CTCCTC GAGGAG(10/8)
BsmBI 496 CGTCTC CGTCTC(1/5)
BspMI 128 ACCTGC ACCTGC(4/8)
BsrDI 642 CATTGC GCAATG(2/0)
BstEII 111 GGTCACC G^GT[ACGT]ACC
BstPI 111 GGTCACC G^GT[ACGT]ACC
BstYI 922 AGATCC [GA]^GATC[CT]
DpnII 923 GATC ^GATC
DraII 896 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 740 CCGTGG C^C[GA][CT]GG
EcoNI 888 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 896 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 111 GGTCACC G^GT[ACGT]ACC
EcoT14I 320 CCTTGG C^C[AT][AT]GG
Fnu4HI 338 GCTGC GC^[ACGT]GC
HapII 60 CCGG C^CGG
HgaI 747 GACGC GACGC(5/10)
HindIII 907 AAGCTT A^AGCTT
HpaII 60 CCGG C^CGG
ItaI 338 GCTGC GC^[ACGT]GC
MaeII 815 ACGT A^CGT
MboI 923 GATC ^GATC
MfeI 824 CAATTG C^AATTG
MflI 922 AGATCC [GA]^GATC[CT]
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 336 CAGCTG C[AC]G^C[GT]G
MspI 60 CCGG C^CGG
MunI 824 CAATTG C^AATTG
MwoI 349 GCAGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 59 CCCGG CC^[GC]GG
NdeII 923 GATC ^GATC
NlaIV 125 GGAACC GG[ACGT]^[ACGT]CC
PmaCI 814 CACGTG CAC^GTG
PmlI 814 CACGTG CAC^GTG
PvuII 336 CAGCTG CAG^CTG
RsaI 105 GTAC GT^AC
SapI 569 GCTCTTC GCTCTTC(1/4)
Sau3AI 923 GATC ^GATC
SexAI 107 ACCTGGT A^CC[AT]GGT
StyI 320 CCTTGG C^C[AT][AT]GG
TseI 338 GCTGC G^C[AT]GC
XhoII 922 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 337 AGCT AG^CT
908 AGCT
AocI 64 CCTGAGG CC^T[ACGT]AGG
900 CCTGAGG
AvaII 69 GGACC G^G[AT]CC
810 GGACC
BsgI 475 CTGCAC GTGCAG(16/14)
595 GTGCAG
BsmFI 48 GGGAC GGGAC(10/14)
235 GTCCC
Bsu36I 64 CCTGAGG CC^T[ACGT]AGG
900 CCTGAGG
EaeI 143 TGGCCA [CT]^GGCC[GA]
374 TGGCCG
EarI 91 CTCTTC CTCTTC(1/4)
570 CTCTTC
Eco81I 64 CCTGAGG CC^T[ACGT]AGG
900 CCTGAGG
Ksp632I 91 CTCTTC CTCTTC(1/4)
570 CTCTTC
SfaNI 243 GATGC GCATC(5/9)
764 GCATC
SfcI 671 CTACAG C^T[GA][CT]AG
867 CTACAG
SmlI 189 CTTGAG C^T[CT][GA]AG
679 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cfr13I DdeI EcoRII
FokI HaeIII HinfI HphI MaeIII
MboII MnlI MslI MvaI NlaIII
PleI Sau96I ScrFI TfiI Tsp45I
Tsp509I TspRI