Restriction map for
pF1KE0893
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII CfoI ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MaeII
MamI MboI MfeI MflI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccII 586 CGCG CG^CG
AflII 19 CTTAAG C^TTAAG
AvaII 921 GGACC G^G[AT]CC
BfrI 19 CTTAAG C^TTAAG
BglI 457 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 690 CTCCAG CTGGAG(16/14)
BsaI 715 GAGACC GGTCTC(1/5)
BsiEI 471 CGACCG CG[GA][CT]^CG
BsmFI 920 GGGAC GGGAC(10/14)
Bsp1407I 941 TGTACA T^GTACA
BsrDI 529 CATTGC GCAATG(2/0)
BsrFI 473 ACCGGC [GA]^CCGG[CT]
BsrGI 941 TGTACA T^GTACA
BstAPI 528 GCATTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 586 CGCG CG^CG
Cfr10I 473 ACCGGC [GA]^CCGG[CT]
DraII 892 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 523 CCACGG C^C[GA][CT]GG
EcoO109I 892 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 319 GATATC GAT^ATC
HindIII 22 AAGCTT A^AGCTT
HinfI 791 GAATC G^A[ACGT]TC
MvnI 586 CGCG CG^CG
SfiI 456 GGCCTCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 19 CTTAAG C^T[CT][GA]AG
SspBI 941 TGTACA T^GTACA
StuI 818 AGGCCT AGG^CCT
TaqI 470 TCGA T^CGA
TfiI 791 GAATC G^A[AT]TC
TthHB8I 470 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 585 CCGC CCGC(-3/-1)
226 GCGG
AcsI 360 GAATTT [GA]^AATT[CT]
1031 GAATTT
AocI 137 CCTCAGG CC^T[ACGT]AGG
984 CCTGAGG
ApoI 360 GAATTT [GA]^AATT[CT]
1031 GAATTT
Asp700I 816 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAACCCTTTC
AspHI 238 GTGCTC G[AT]GC[AT]^C
784 GTGCTC
BalI 464 TGGCCA TGG^CCA
482 TGGCCA
BcnI 249 CCCGG CC^[GC]GG
924 CCGGG
BmyI 238 GTGCTC G[AGT]GC[ACT]^C
784 GTGCTC
BsiHKAI 238 GTGCTC G[AT]GC[AT]^C
784 GTGCTC
Bsp1286I 238 GTGCTC G[AGT]GC[ACT]^C
784 GTGCTC
BstXI 101 CCAGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
692 CCAGCCAGATGG
Bsu36I 137 CCTCAGG CC^T[ACGT]AGG
984 CCTGAGG
EarI 162 CTCTTC CTCTTC(1/4)
1002 GAAGAG
Eco81I 137 CCTCAGG CC^T[ACGT]AGG
984 CCTGAGG
HphI 357 GGTGA GGTGA(8/7)
705 GGTGA
Ksp632I 162 CTCTTC CTCTTC(1/4)
1002 GAAGAG
MluNI 464 TGGCCA TGG^CCA
482 TGGCCA
MscI 464 TGGCCA TGG^CCA
482 TGGCCA
MseI 20 TTAA T^TAA
632 TTAA
NciI 249 CCCGG CC^[GC]GG
924 CCGGG
PflMI 696 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCAGGAAGTGG
SfaNI 351 GATGC GCATC(5/9)
560 GCATC
Tru9I 20 TTAA T^TAA
632 TTAA
Van91I 696 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCAGGAAGTGG
XmnI 816 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAACCCTTTC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BsmAI BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HpaII ItaI MaeIII MboII MnlI
MslI MspI MvaI MwoI NlaIII
NlaIV PstI RsaI Sau96I ScrFI
SfcI TseI Tsp45I Tsp509I TspRI
XcmI