Restriction map for
pF1KE0887
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 404 GTCTAC GT^[AC][GT]AC
AclI 772 AACGTT AA^CGTT
AcsI 1040 AAATTC [GA]^AATT[CT]
ApoI 1040 AAATTC [GA]^AATT[CT]
Asp700I 771 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 619 TGCGCA TGC^GCA
BalI 485 TGGCCA TGG^CCA
BbsI 1008 GTCTTC GAAGAC(2/6)
BcgI 536 GCATCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 515 TGATCA T^GATCA
BpuAI 1008 GTCTTC GAAGAC(2/6)
BsaAI 481 TACGTG [CT]AC^GT[GA]
BsaBI 708 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 678 CTGCAC GTGCAG(16/14)
Bsp1407I 944 TGTACA T^GTACA
BspMI 832 ACCTGC ACCTGC(4/8)
BsrGI 944 TGTACA T^GTACA
BssSI 544 CTCGTG CACGAG(-5/-1)
BstXI 695 CCAATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 620 GCGC GCG^C
Cfr13I 915 GGCCC G^G[ACGT]CC
DsaI 851 CCGTGG C^C[GA][CT]GG
EaeI 485 TGGCCA [CT]^GGCC[GA]
EarI 862 CTCTTC CTCTTC(1/4)
FbaI 515 TGATCA T^GATCA
FspI 619 TGCGCA TGC^GCA
HhaI 620 GCGC GCG^C
HinP1I 620 GCGC G^CGC
Ksp632I 862 CTCTTC CTCTTC(1/4)
MamI 708 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
MslI 738 CATTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 172 CAGCTG C[AC]G^C[GT]G
NdeI 489 CATATG CA^TATG
NlaIV 992 GGAACC GG[ACGT]^[ACGT]CC
Psp1406I 772 AACGTT AA^CGTT
PstI 834 CTGCAG CTGCA^G
PvuII 172 CAGCTG CAG^CTG
Sau96I 915 GGCCC G^G[ACGT]CC
SspBI 944 TGTACA T^GTACA
SspI 35 AATATT AAT^ATT
TaqI 781 TCGA T^CGA
TfiI 969 GAATC G^A[AT]TC
TthHB8I 781 TCGA T^CGA
XmnI 771 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 460 GCGG CCGC(-3/-1)
816 GCGG
BanII 522 GAGCCC G[GA]GC[CT]^C
529 GGGCTC
BbvI 171 GCAGC GCAGC(8/12)
836 GCAGC
BmyI 522 GAGCCC G[AGT]GC[ACT]^C
529 GGGCTC
BpmI 175 CTGGAG CTGGAG(16/14)
442 CTGGAG
BsaI 724 GGTCTC GGTCTC(1/5)
855 GGTCTC
BseRI 241 CTCCTC GAGGAG(10/8)
178 GAGGAG
BsmAI 725 GTCTC GTCTC(1/5)
856 GTCTC
BsmFI 871 GGGAC GGGAC(10/14)
346 GTCCC
BsmI 567 GAATGC GAATGC(1/-1)
448 GCATTC
Bsp1286I 522 GAGCCC G[AGT]GC[ACT]^C
529 GGGCTC
BspHI 518 TCATGA T^CATGA
563 TCATGA
BsrI 30 ACTGG ACTGG(1/-1)
718 ACTGG
BstF5I 554 CATCC GGATG(2/0)
786 CATCC
Fnu4HI 171 GCAGC GC^[ACGT]GC
836 GCAGC
FokI 554 CATCC GGATG(9/13)
786 CATCC
ItaI 171 GCAGC GC^[ACGT]GC
836 GCAGC
MseI 88 TTAA T^TAA
1024 TTAA
MwoI 174 GCTGGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
460 GCGGTCATGGC
RcaI 518 TCATGA T^CATGA
563 TCATGA
Tru9I 88 TTAA T^TAA
1024 TTAA
TseI 171 GCAGC G^C[AT]GC
836 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AlwI BfaI BsaJI
BsiYI BslI BssKI BstNI BstYI
Cac8I DdeI DpnII EcoRII HaeIII
HinfI HphI MaeI MaeII MaeIII
MboI MboII MflI MnlI MvaI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI SfaNI SfcI Tsp45I Tsp509I
TspRI XhoII