Restriction map for
pF1KE0885
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 593 ACATGT A^C[GA][CT]GT
AlwNI 127 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 906 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 181 GTGCTC G[AT]GC[AT]^C
AvaI 566 CTCGAG C^[CT]CG[GA]G
BcnI 952 CCGGG CC^[GC]GG
BglI 400 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 710 TACGTA [CT]AC^GT[GA]
BsgI 217 CTGCAC GTGCAG(16/14)
BsiHKAI 181 GTGCTC G[AT]GC[AT]^C
BsoBI 566 CTCGAG C^[CT]CG[GA]G
Bsp1407I 596 TGTACA T^GTACA
BspHI 692 TCATGA T^CATGA
BspLU11I 593 ACATGT A^CATGT
BsrGI 596 TGTACA T^GTACA
Cfr13I 562 GGGCC G^G[ACGT]CC
EcoNI 974 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 371 CCTTGG C^C[AT][AT]GG
EcoT22I 940 ATGCAT ATGCA^T
HapII 952 CCGG C^CGG
HgaI 813 GACGC GACGC(5/10)
HpaII 952 CCGG C^CGG
MaeII 711 ACGT A^CGT
MspI 952 CCGG C^CGG
NciI 952 CCGGG CC^[GC]GG
NlaIV 948 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 940 ATGCAT ATGCA^T
PaeR7I 566 CTCGAG C^TCGAG
PstI 832 CTGCAG CTGCA^G
RcaI 692 TCATGA T^CATGA
Sau96I 562 GGGCC G^G[ACGT]CC
SexAI 158 ACCTGGT A^CC[AT]GGT
SfaNI 294 GATGC GCATC(5/9)
SmlI 566 CTCGAG C^T[CT][GA]AG
SnaBI 710 TACGTA TAC^GTA
SspBI 596 TGTACA T^GTACA
StuI 982 AGGCCT AGG^CCT
StyI 371 CCTTGG C^C[AT][AT]GG
TaqI 567 TCGA T^CGA
TfiI 208 GATTC G^A[AT]TC
TthHB8I 567 TCGA T^CGA
XhoI 566 CTCGAG C^TCGAG
XmnI 906 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 202 AGCT AG^CT
353 AGCT
AocI 683 CCTTAGG CC^T[ACGT]AGG
924 CCTGAGG
BsmFI 811 GGGAC GGGAC(10/14)
312 GTCCC
BsrI 658 ACTGG ACTGG(1/-1)
309 CCAGT
BstEII 375 GGTGACC G^GT[ACGT]ACC
549 GGTGACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
549 GGTGACC
BstXI 152 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
290 CCAAGATGCTGG
Bsu36I 683 CCTTAGG CC^T[ACGT]AGG
924 CCTGAGG
Cac8I 612 GCTGGC GC[ACGT]^[ACGT]GC
816 GCTTGC
Eco81I 683 CCTTAGG CC^T[ACGT]AGG
924 CCTGAGG
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
549 GGTGACC
MseI 11 TTAA T^TAA
31 TTAA
MwoI 400 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
646 GCGTTGATTGC
SfcI 832 CTGCAG C^T[GA][CT]AG
918 CTACAG
Tru9I 11 TTAA T^TAA
31 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AfaI AlwI BalI BanII BbvI
BmyI BsaI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstF5I BstNI BstYI DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MvaI NdeII
NlaIII NspI PleI RsaI Sau3AI
ScrFI TseI Tsp45I TspRI XhoII