Restriction map for
pF1KE0884
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 772 GTATAC GT^[AC][GT]AC
AocI 870 CCTGAGG CC^T[ACGT]AGG
AseI 580 ATTAAT AT^TAAT
AsnI 580 ATTAAT AT^TAAT
Asp700I 852 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 127 GTGCTC G[AT]GC[AT]^C
AvaII 447 GGTCC G^G[AT]CC
BamHI 74 GGATCC G^GATCC
BbsI 902 GAAGAC GAAGAC(2/6)
BbvCI 552 GCTGAGG CCTCAGC(-5/-2)
BclI 641 TGATCA T^GATCA
BfaI 910 CTAG C^TAG
BpmI 253 CTCCAG CTGGAG(16/14)
BpuAI 902 GAAGAC GAAGAC(2/6)
BsaI 312 GGTCTC GGTCTC(1/5)
BseRI 481 CTCCTC GAGGAG(10/8)
BsiHKAI 127 GTGCTC G[AT]GC[AT]^C
BspHI 485 TCATGA T^CATGA
BsrI 255 CCAGT ACTGG(1/-1)
Bst1107I 772 GTATAC GTA^TAC
BstYI 74 GGATCC [GA]^GATC[CT]
BstZ17I 772 GTATAC GTA^TAC
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
Cac8I 161 GCCTGC GC[ACGT]^[ACGT]GC
DraII 446 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 586 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
EcoO109I 446 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 317 CCTTGG C^C[AT][AT]GG
EcoT22I 886 ATGCAT ATGCA^T
FauI 158 CCCGC CCCGC(4/6)
FbaI 641 TGATCA T^GATCA
MaeI 910 CTAG C^TAG
MflI 74 GGATCC [GA]^GATC[CT]
MspA1I 70 CAGCGG C[AC]G^C[GT]G
MwoI 346 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 356 CATATG CA^TATG
NsiI 886 ATGCAT ATGCA^T
PpuMI 446 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 580 ATTAAT AT^TAAT
RcaI 485 TCATGA T^CATGA
SexAI 104 ACCTGGT A^CC[AT]GGT
SfaNI 240 GATGC GCATC(5/9)
SfcI 864 CTACAG C^T[GA][CT]AG
StyI 317 CCTTGG C^C[AT][AT]GG
XhoII 74 GGATCC [GA]^GATC[CT]
XmnI 852 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 102 GTAC GT^AC
177 GTAC
AlwI 75 GATCC GGATC(4/5)
74 GGATC
BanII 408 GAGCCC G[GA]GC[CT]^C
893 GGGCTC
BsgI 163 CTGCAC GTGCAG(16/14)
737 GTGCAG
BsmFI 757 GGGAC GGGAC(10/14)
258 GTCCC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCAAGATGCTGG
Cfr13I 324 GGCCC G^G[ACGT]CC
447 GGTCC
DpnII 75 GATC ^GATC
642 GATC
EarI 211 CTCTTC CTCTTC(1/4)
748 CTCTTC
Ksp632I 211 CTCTTC CTCTTC(1/4)
748 CTCTTC
MboI 75 GATC ^GATC
642 GATC
MseI 581 TTAA T^TAA
923 TTAA
NdeII 75 GATC ^GATC
642 GATC
NlaIV 74 GGATCC GG[ACGT]^[ACGT]CC
446 GGGTCC
NspI 562 GCATGT [GA]CATG^[CT]
884 ACATGC
RsaI 102 GTAC GT^AC
177 GTAC
Sau3AI 75 GATC ^GATC
642 GATC
Sau96I 324 GGCCC G^G[ACGT]CC
447 GGTCC
Tru9I 581 TTAA T^TAA
923 TTAA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BalI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI DdeI EaeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboII MluNI
MnlI MscI MslI MvaI NlaIII
PleI ScrFI TfiI TseI Tsp45I
TspRI