Restriction map for
pF1KE0881
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 571 GACGTC GACGT^C
AciI 777 CCGC CCGC(-3/-1)
AcyI 571 GACGTC G[GA]^CG[CT]C
AocI 235 CCTAAGG CC^T[ACGT]AGG
Asp700I 900 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 464 GTGCTC G[AT]GC[AT]^C
AvrII 600 CCTAGG C^CTAGG
BciVI 422 GTATCC GTATCC(6/5)
BlnI 600 CCTAGG C^CTAGG
BmyI 464 GTGCTC G[AGT]GC[ACT]^C
BsaHI 571 GACGTC G[GA]^CG[CT]C
BseRI 921 CTCCTC GAGGAG(10/8)
BsgI 711 CTGCAC GTGCAG(16/14)
BsiEI 360 CGATCG CG[GA][CT]^CG
BsiHKAI 464 GTGCTC G[AT]GC[AT]^C
BsmAI 643 GTCTC GTCTC(1/5)
Bsp1286I 464 GTGCTC G[AGT]GC[ACT]^C
BspHI 308 TCATGA T^CATGA
BsrDI 349 GCAATG GCAATG(2/0)
Bsu36I 235 CCTAAGG CC^T[ACGT]AGG
Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 361 GATC ^GATC
DsaI 412 CCACGG C^C[GA][CT]GG
EarI 904 CTCTTC CTCTTC(1/4)
Eco81I 235 CCTAAGG CC^T[ACGT]AGG
EcoT22I 633 ATGCAT ATGCA^T
Hin1I 571 GACGTC G[GA]^CG[CT]C
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
HphI 447 GGTGA GGTGA(8/7)
Ksp632I 904 CTCTTC CTCTTC(1/4)
MaeII 572 ACGT A^CGT
MboI 361 GATC ^GATC
MspA1I 378 CAGCTG C[AC]G^C[GT]G
NdeII 361 GATC ^GATC
NlaIV 721 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 633 ATGCAT ATGCA^T
NspI 767 GCATGT [GA]CATG^[CT]
PflMI 252 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 360 CGATCG CGAT^CG
PvuII 378 CAGCTG CAG^CTG
RcaI 308 TCATGA T^CATGA
Sau3AI 361 GATC ^GATC
SpeI 52 ACTAGT A^CTAGT
TaqI 363 TCGA T^CGA
TfiI 917 GAATC G^A[AT]TC
TthHB8I 363 TCGA T^CGA
Van91I 252 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 900 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 597 GTAC GT^AC
755 GTAC
BbsI 76 GTCTTC GAAGAC(2/6)
670 GTCTTC
BpuAI 76 GTCTTC GAAGAC(2/6)
670 GTCTTC
BsiYI 195 CCTCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
252 CCATCTCTTGG
BslI 195 CCTCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
252 CCATCTCTTGG
DdeI 236 CTAAG C^T[ACGT]AG
868 CTGAG
MslI 577 CACTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
626 CATTACTATG
PleI 408 GAGTC GAGTC(4/5)
693 GAGTC
RsaI 597 GTAC GT^AC
755 GTAC
SfcI 861 CTATAG C^T[GA][CT]AG
895 CTACAG
Tsp45I 536 GTGAC ^GT[GC]AC
835 GTGAC
XcmI 191 CCAACCTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
248 CCAACCATCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BalI BbvI BfaI BsaJI
BsrI BssKI BstF5I BstNI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboII
MluNI MnlI MscI MseI MvaI
MwoI NlaIII ScrFI SfaNI StyI
Tru9I TseI Tsp509I TspRI